Project description:Masson pine (Pinus massoniana) has evolved some adaptations for growth in low P soils. To elucidate these mechanisms, we investigated global gene expression profiles of the masson pine responding to long-term phosphorus starvation and different Pi levels (P1, 0.01 mM P; P2, 0.06 mM P).
Project description:Masson pine (Pinus massoniana) has evolved some adaptations for growth in low P soils. To elucidate these mechanisms, we investigated global gene expression profiles of the masson pine responding to long-term phosphorus starvation and different Pi levels (P1, 0.01 mM P; P2, 0.06 mM P). Analysis used phosphorus-sufficient treatment RNA as control samples for comparison to the experimental samples (P1 and P2) taken at 12, 24, 48 and 60 day. Indirect comparisons were made across multiple arrays with raw data pulled from different channels for data analysis and comparison to the control data.
Project description:The wild grass Holcus lanatus L., an outcrossing diploid (2n=14) and closely related to B. distachyon (Aliscioni et al., 2012), has a remarkable balanced polymorphism in arsenate tolerance, screened from a semi-natural, non-arsenic contaminated populations (Meharg et al., 1993), coded by a single gene (Macnair et al., 1992). As arsenate is a phosphate analogue it has been postulated that this polymorphism is maintained due to phosphorus nutrition, not arsenate tolerance per se, particularly as the tolerance gene co-segregates with suppression of High affinity Phosphate Transport (HAPT) (Meharg et al., 1992a; Meharg & Macnair, 1992b), though an explicit ecological link to phosphorus status of soils has yet to be proven (Naylor et al., 1996). The aim of this study is to address soil phosphate responsiveness (+/-) along with the transcriptomic consequences of being of arsenic tolerant (T) or non-tolerant (N) phenotype to ascertain why and how this polymorphism is maintained.