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Full-length circRNAs in IMR-90 and A549 cell lines using nanopore sequencing

ABSTRACT: Full-length circRNAs in IMR-90 and A549 cell lines using nanopore sequencing

PROVIDER: PRJNA1091574 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28437163_1.fastq.gz	Fastqsanger.gz
	SRR28437164_1.fastq.gz	Fastqsanger.gz
	SRR28437165_1.fastq.gz	Fastqsanger.gz
	SRR28437166_1.fastq.gz	Fastqsanger.gz

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Full-length circRNAs in IMR-90 and A549 cell lines using nanopore sequencing

Project description:To identify full-length circRNAs in lung normal and cancer cell lines, we enriched circRNAs by rRNA depletion, poly(A) tailing and Rnase R treatment and amplified circRNAs by rolling circular RT. We constructed library according to the protocol of Fangqing Zhao team's. Nanopore is used for full-length sequencing.

2025-05-27 | GSE262357 | GEO

Phosphoproteome of cyclic stretch A549 and IMR-90

Project description:Lung cancer is one of the leading causes of death. However, most of the researches were based on the traditional cell-culturing method. Whereas cells of lung are subjected to the mechanical forces periodically while breathing. In the present study, we applied cyclic stretch to stimulate the continuously contracting physical condition. We uncovered the stretching force-induced phosphoproteome in lung cancer cell A549 and fibroblast IMR-90. 2048 and 2604 phosphosites corresponding to 837 and 1008 phosphoproteins were identified in A549 and IMR-90, respectively. Interestingly, cytoskeleton reorganization and mitochondrial localization were enriched in the significantly expressed phosphoproteins in response to cyclic stretch. Indeed, we found this physical stress changed cell alignment thus disrupted mitochondrial dynamics. We proved that mitochondrial fusion is induced by uniaxial stretch in 2 cell lines. This study reveals the molecular mechanism of cyclic stretch and supports that stretching force enhanced cellular rearrangement and mitochondrial fusion in lung cells.

2021-09-08 | PXD012010 | Pride

ChIP-seq from IMR-90 (ENCSR970FKL)

Project description:ChIP-seq on human IMR-90 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-01-04 | GSE92197 | GEO

ChIP-seq from IMR-90 (ENCSR664OKA)

Project description:ELK1 ChIP-seq on human IMR-90 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-01-04 | GSE91969 | GEO

ChIP-seq from IMR-90 (ENCSR124AIG)

Project description:FOS ChIP-seq on human IMR-90 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-10-20 | GSE105300 | GEO

Sequencing of human IMR-90 at 27 and 55 population doublings

Project description:Comparisong of gene expression of human IMR-90 at 27 and 55 population doublings. RNA-seq data comprises 2 groups: 27 and 55 population doublings of IMR-90. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)

2021-10-19 | GSE117813 | GEO

microRNA-seq from IMR-90 (ENCSR123QKC)

Project description:RNA Evaluation IMR-90 microRNA-seq from Mortazavi For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2021-06-15 | GSE176683 | GEO

intact Hi-C from IMR-90 (ENCSR345VTI)

Project description:IMR-90 intact Hi-C For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2023-07-24 | GSE237347 | GEO

RNA-Seq in retinoblastoma 1 (RB1) knock out IMR-90 cells

Project description:Total RNA-seq with rRNA depletion in retinoblastoma protein 1 knockout (RB1 KO) IMR-90

2021-12-29 | GSE173127 | GEO

IMR-90 cells: Collagen Mesh Time Course

Project description:A series of chips with some repeated measurments. Each chip represents a pool of 4 collagen/chondroitin sulfate tissue engineering scaffold meshes seeded with 1 x 10^6 IMR-90 Human Fibroblasts. mRNA was isolated at 30 minutes, 1, 2, 4, 8, 12, 24, 48, and 72 hours after seeding. Keywords = tissue engineering mesh, collagen, chondroitin sulfate, biomaterials design Keywords: time-course

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