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Overexpression of miR-200c/141 and miR-200b/200a/429 in MDA-MB-436 cells

ABSTRACT: Overexpression of miR-200c/141 and miR-200b/200a/429 in MDA-MB-436 cells

PROVIDER: PRJNA1091958 | ENA |

REPOSITORIES: ENA

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Overexpression of miR-200c/141 and miR-200b/200a/429 in MDA-MB-436 cells

Project description:We previously showed that overexpression of the miR-200c/141 cluster in the TNBC cell line MDA-MB-231 significantly reduced proliferation and invasion in vitro and tumor growth and metastasis in vivo. To confirm the effects of miR-200s in another TNBC cell line, MDA-MB-436 cells were created to overexpress both the miR-200c/141 cluster and the miR-200b/200a/429 cluster.

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Transgenic overexpression of the miR-200b/200a/429 cluster prevents mammary tumor development in MTB-TAN mice

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Searching for target genes of miR-429 in HCCLM3 cells

Project description:miRNAs are an class of small noncoding RNAs and about 21-25 nucleotides in length. miRNAs inhibit the translation or induce mRNA degradation by binding to the 3’ UTR of target mRNAs and have been identified as the tumor promoters or suppressors regulating the progression of cancers. miR-429, which is a member of an evolutionarily conserved family of miRNAs that includes miR-200b, miR-200a, miR-200c and miR-141, is expressed in various epithelial tissues. Our goal is to search the possible target genes of miR-429 in human liver cancer cell line HCCLM3. We analyzed possible target genes of miR-429 by identifying the shared genes among the down-regulated genes in epithelial cell adhesion molecule (EpCAM) negative HCCLM3 cells which treated with miR-429 mimics and the up-regulated genes in EpCAM positive HCCLM3 cells which treated with Antagomir-429.

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Transgenic overexpression of the miR-200b/200a/429 cluster inhibits mammary tumor initiation.

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