Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1097528 | ENA |
REPOSITORIES: ENA
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Project description:The urine of bank voles (Myodes glareolus) contains substantial quantities of a small protein that is expressed at much higher levels in males than females, and at higher levels in males in the breeding season. This protein was purified and completely sequenced at the protein level by mass spectrometry. Leucine/isoleucine ambiguity was completely resolved by metabolic labelling, monitoring the incorporation of dietary deuterated leucine into specific sites in the protein. The predicted mass of the sequenced protein was exactly consonant with the mass of the protein measured in bank vole urine samples, correcting for the formation of two disulphide bonds. The sequence of the protein revealed that it was a lipocalin related to aphrodisin and other odorant binding proteins (OBPs), but differed from all OBPs previously described. The pattern of secretion in urine used for scent marking by male bank voles, and similarity to other lipocalins used as chemical signals in rodents, suggest that this protein plays a role in male sexual and/or competitive communication. We propose the name glareosin for this novel protein to reflect the origin of the protein and to emphasise the distinction from known OBPs.
2017-09-13 | PXD006645 | Pride
Project description:We use ChIP-seq targeting histone 3 lysine 27-acetylation (H3K27ac) to identify putative enhancer sites genome-wide in the ventral pallidum cortex of adult prairie voles
2018-01-31 | GSE85826 | GEO
Project description:Bank voles are susceptible to prion strains from many different species, yet the molecular mechanisms underlying the ability of bank vole prion protein (BVPrP) to function as a universal prion acceptor remain unclear. Potential differences in molecular environments and protein interaction networks on the cell surface of brain cells may contribute to BVPrP’s unusual behavior. To test this hypothesis, we generated knock-in mice that express physiological levels of BVPrP (M109 isoform) and employed mass spectrometry to compare the interactomes of mouse (Mo) PrP and BVPrP following mild in vivo crosslinking of brain tissue. Substantial overlap was observed between the top interactors for BVPrP and MoPrP, with established PrP-interactors such as neural cell adhesion molecules, subunits of Na+/K+-ATPases, and contactin-1 being equally present in the two interactomes. We conclude that the molecular environments of BVPrP and MoPrP in the brains of mice are very similar. This suggests that the unorthodox properties of BVPrP are unlikely to be mediated by differential interactions with other proteins.
2026-07-06 | PXD059662 | Pride
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
| 2533916 | ecrin-mdr-crc
Project description:This study aimed to uncover changes in gene expression in key brain regions associated with social bonding neuroplasticity in adult prairie voles. The experimental workflow involved the following steps: 1. Male (Ma) and female (Fe) prairie voles were subjected to social cohabitation and mating (SCM) conditions for 120 h, with isolated animals serving as controls. 2. RNA was extracted and analyzing RNA from three brain regions crucial for social bonding and neuroplasticity: the subventricular zone (SVZ), dentate gyrus (DG), and nucleus accumbens (NAc). 3. RNA sequencing was performed to identify differentially expressed genes between the SCM and control groups. 4. Gene ontology (GO) and functional enrichment analyses were performed to understand the biological processes involved in pair bonding. 5. Validation of key findings through quantitative PCR and in vitro neurosphere assays using cells isolated from the SVZ. This approach allowed the examination of sex-specific changes in gene expression related to neurogenesis, synaptic plasticity, and other probable molecular pathways involved in pair bond formation, with potential implications for understanding human relationships and related neuropsychiatric conditions.
2025-05-15 | E-MTAB-15128 | biostudies-arrayexpress
Project description:The intent of the experiment was to infer, from re-sequencing of genomic DNA, the coordinates of neo-insertions from activated ONSEN/COPIA78 LTR retrotrasnposon in Arabidopsis thaliana. For this, we performed Illumina 75 bp pair-end PCR-free DNA genome re-sequencing in several independent lines of RdDM mutant nrpd1-3.
2018-03-18 | E-MTAB-5883 | biostudies-arrayexpress