Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparative analysis of the effects of various chamomile extracts and characteristic pure compounds on human T cells

ABSTRACT: comparative analysis of the effects of various chamomile extracts and characteristic pure compounds on human T cells

PROVIDER: PRJNA1097545 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

comparative analysis of the effects of various chamomile extracts and characteristic pure compounds on human T cells

Project description:In this study, we have characterized and compared the effects of differently prepared chamomile extracts and characteristic pure compounds on the T cell redox milieu as well as on the migration, activation, proliferation, and cytokine production of primary human T cells. Futhermore, nCounter based gene expression profiling was performed on the most promising extracts (Chamomile aqueous total fermented-CT, aqueous toot fermented-CR, and ethanolic flower-CF) and pure compounds (Apigenin-Ap and Chamazulene-Cz) to identify the genes related to different T cell functions, that were targeted by the different treatment conditions.

2025-04-08 | GSE263440 | GEO

Estrogenic activity of fermented soymilk extracts and soy compounds

Project description:Estrogenic activity of fermented soymilk extracts and soy compounds

| PRJNA940837 | ENA

Urease Inhibitory Kinetic Studies of Various Extracts and Pure Compounds from Cinnamomum Genus.

Project description:Urease is an enzyme that plays a significant role in the hydrolysis of urea into carbonic acid and ammonia via the carbamic acid formation. The resultant increase in pH leads to the onset of various pathologies such as gastric cancer, urolithiasis, hepatic coma, hepatic encephalopathy, duodenal ulcers and peptic ulcers. Urease inhibitors can reduce the urea hydrolysis rate and development of various diseases. The Cinnamomum genus is used in a large number of traditional medicines. It is well established that stem bark of Cinnamomum cassia exhibits antiulcerogenic potential. The present study evaluated the inhibitory effect of seven extracts of Cinnamomum camphora, Cinnamomum verum and two pure compounds Camphene and Cuminaldehyde on urease enzyme. Kinetic studies of potential inhibitors were carried out. Methanol extract (IC50 980 µg/mL) of C. camphora and a monoterpene Camphene (IC50 0.147 µg/mL) possess significant inhibitory activity. The Lineweaver Burk plot analysis suggested the competitive inhibition by methanol extract, hexane fraction and Camphene. The Gas Chromatography-Mass Spectroscopy (GC-MS) analysis of hexane fraction revealed the contribution of various terpenes. The present study targets terpenes as a new class of inhibitors that have potential therapeutic value for further development as novel drugs.

| S-EPMC8270325 | biostudies-literature

Estrogenic activity of fermented soymilk extracts and soy compounds

Project description:To understand the complex mechanism of estrogen action, multiple assays based on different biological principles is important. Here, multiple assays based on cell, protein and transcription assays were used to evaluate estrogenic activity of soymilk extracts before and after fermentation, and four soy compounds, genistein and daidzein, and their glycosides, genistin and daidzin, respectively. The data obtained by a transcription assay, RNA-sequencing (RNA-seq), was further examined by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database analysis to understand cell functions, i.e. biological processes and metabolic pathways. Among the cell functions identified, the sets of genes for cell division/cell cycle and DNA replication/DNA repair are significantly up-regulated, whereas those for autophagy are significantly down-regulated, which explains well the enhanced activity of cell proliferation by these materials. The applications of estrogenic activity of soymilk extracts and soy flavonoids, and the contribution of fermentation, include pharmacological benefits, such as bone protection/bone regeneration, cancer chemoprevention, neuroprotection and the treatment of menopausal syndromes.

2023-10-31 | GSE226627 | GEO

Pure and mixed crude extracts of Thauera aromatica K172 and Pseudomonas putida KT2440 for benchmarking GroEL-proteotyping

Project description:This dataset has been used to establish GroEL-proteotyping, a targeted proteotyping approach for assessing bacterial community compositions using the taxonomic marker protein GroEL. This dataset contains raw data of two experiments: 1. Pure cultures of T. aromatica (sample names: TA, TB, TC) and P. putida (sample names: PA, PB, PC) were analyzed individually after in-solution digestion 2. T. aromatica and P. putida were cultivated independently and crude extracts were mixed in defined ratios. GroEL was pre-separated by SDS-PAGE (sample names: LOD_..., in-gel digestion)

2023-10-27 | PXD046460 | Pride

TMT-10plex-based phosphoproteomics of bioactive compounds in A549 cells

Project description:We conducted a comprehensive screening of bioactive compounds sourced from natural products, specifically targeting those capable of inducing apoptosis while inhibiting tyrosine kinase activity. Our investigation encompassed a diverse range of compounds, including pure compounds, compound mixtures, and peptides, all evaluated within the A549 cell line. To delve into the underlying molecular mechanisms, we employed phosphoproteomics analysis, which enabled us to comprehensively assess the impact of these bioactive compounds on cellular pathways. Through labeling digested proteins with distinct isobaric tags, we meticulously examined the cellular response to each compound. In our experimental design, we included two established chemical drugs, Afatinib and Osimertinib, serving as positive controls. These drugs, both kinase inhibitors utilized in cancer treatment, operate via distinct mechanisms and target different kinases. By comparing the effects of our test compounds with these controls, we aimed to elucidate their potential therapeutic relevance and mechanisms of action. Among the compounds examined were extracts from Phallus indusiatus and Fomes rimosus (Berk.) Cooke, as well as specific compounds like Chamuangone, Cannabigerol (CBG), Cannabidiol (CBD), and NP1-cyclic peptide

2024-03-13 | PXD050555 | Pride

Clarion GO screen assay to assess gene expression responses of human SK-N-SH neuroblastoma cells under control and IL-1B induced conditions in response to herbal extracts of Sage (S. offinicalis) and Chamomile (C. nobile).

Project description:Chronic inflammation plays a critical role in the initiation and development of various human illnesses. The use of steroidal anti-inflammatory drugs (SAIDs) or non-steroidal anti-inflammatory drugs (NSAIDs) is now much more frequent and presents a number of unwanted side effects. For example long- or short-term usage of SAIDs presents multiple negative side effects such as stomach irritation, thinning of the skin, immune defence regression, weight gain and even sometimes a cortico-dependence, and NSAIDs have been linked to a higher risk of strokes, heart attacks, and heart-related deaths. In parallel, there has been renewed interest in alternative medicines and natural therapies and thousands of potential medicinal plants, including sage and chamomile. This study assesses the gene expression responses of human SK-N-SH neuroblastoma cells, pre-treated with aqueous ethanol extracts of sage (Salvia officinalis) or Roman chamomile (Chamaemelum nobile), following 4h or 24h treatment with IL-1B versus control conditions.

2020-10-05 | E-MTAB-9611 | biostudies-arrayexpress

init_30_test_portion with various compounds

Project description:Mass spectra of various compounds registered using Thermo Focus DSQ EI instrument without turning off the filament

2021-04-12 | MSV000087180 | MassIVE

Effects of digested onion extracts on intestinal gene expression: an interspecies comparison using different intestine models

Project description:Applicability of in vitro (human Caco-2 cells) and ex vivo intestine models (rat precision cut intestine slices and the pig in-situ small intestinal segment perfusion (SISP) technique) to study the effect of food compounds. In vitro digested yellow (YOd) and white onion extracts (WOd) were used as model food compounds and transcriptomics was applied to obtain more insight into which extent mode of actions depend on the model.

2016-09-16 | GSE83908 | GEO

Methylophilus sp. 'Pure River'

Project description:Methylophilus sp. 'Pure River' Genome sequencing

| PRJNA1180274 | ENA