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Conservation of Regulatory Elements with Highly Diverged Sequences Across Large Evolutionary Distances [RNA-seq]

ABSTRACT: Conservation of Regulatory Elements with Highly Diverged Sequences Across Large Evolutionary Distances [RNA-seq]

PROVIDER: PRJNA1098214 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28602662_1.fastq.gz	Fastqsanger.gz
	SRR28602662_2.fastq.gz	Fastqsanger.gz
	SRR28602663_1.fastq.gz	Fastqsanger.gz
	SRR28602663_2.fastq.gz	Fastqsanger.gz
	SRR28602664_1.fastq.gz	Fastqsanger.gz

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Project description:BACKGROUND: In today's age of genomic discovery, no attempt has been made to comprehensively sequence a gymnosperm genome. The largest genus in the coniferous family Pinaceae is Pinus, whose 110-120 species have extremely large genomes (c. 20-40 Gb, 2N = 24). The size and complexity of these genomes have prompted much speculation as to the feasibility of completing a conifer genome sequence. Conifer genomes are reputed to be highly repetitive, but there is little information available on the nature and identity of repetitive units in gymnosperms. The pines have extensive genetic resources, with approximately 329000 ESTs from eleven species and genetic maps in eight species, including a dense genetic map of the twelve linkage groups in Pinus taeda. RESULTS: We present here the Sanger sequence and annotation of ten P. taeda BAC clones and Genome Analyzer II whole genome shotgun (WGS) sequences representing 7.5% of the genome. Computational annotation of ten BACs predicts three putative protein-coding genes and at least fifteen likely pseudogenes in nearly one megabase of sequence. We found three conifer-specific LTR retroelements in the BACs, and tentatively identified at least 15 others based on evidence from the distantly related angiosperms. Alignment of WGS sequences to the BACs indicates that 80% of BAC sequences have similar copies (> or = 75% nucleotide identity) elsewhere in the genome, but only 23% have identical copies (99% identity). The three most common repetitive elements in the genome were identified and, when combined, represent less than 5% of the genome. CONCLUSIONS: This study indicates that the majority of repeats in the P. taeda genome are 'novel' and will therefore require additional BAC or genomic sequencing for accurate characterization. The pine genome contains a very large number of diverged and probably defunct repetitive elements. This study also provides new evidence that sequencing a pine genome using a WGS approach is a feasible goal.

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Conservation of Regulatory Elements with Highly Diverged Sequences Across Large Evolutionary Distances [RNA-seq]

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