Project description:Chromatin accessibility mapping by DNase-seq on FACS-isolated cell populations during Drosophila melanogaster embryogenesis (6-8 hrs after egg-laying)
Project description:In this study we aimed to obtain gene expression data during development of Drosophila melanogaster. Therefore 10 critical stages from egg to adult fly were chosen. Specifically the purpose of the project was to study gene expression in the under-replicated regions of polytene chromosomes that were discovered earlier (see GSE2551).
Project description:In this study we use a combination of proteomics Label-Free quantification methods to monitor protein expression changes over a time course of more than 20 hours of embryo development in Drosophila melanogaster.
Project description:Genomewide mapping of Drosophila Lmd protein binding during embryonic development. Two consecutive timepoints (6-8 and 8-10 hrs after egg-laying) were assayed in four independent repeats each. Two different antibodies were used to precipitate the Lmd protein. Additionally the respective preimmune-serum was used as a control for every precipitation. The enriched DNA was hybridized to tiling arrays covering ~ 50% of the Drosophila melanogaster genome.
