Project description:The transcriptome profiling five tissues of juvenile Eriocheir sinensis, including gill, muscle, thoracic ganglion, eyestalk and hepatopancreas, were sequenced to get the basic dataset for constructed a genome-scale metabolic network model for E. sinensis. The model was used to predict the optimal nutrient requirements of E. sinensis in feed and suggestions for feed improvement were put forward based on the simulation results.
Project description:Reference genome was assembed for mixture sample of Eriocheir sinensis eyestalk under low tempeture (7°C,12°C,17°C) ,normal temperature (22°C) and high temperature (27°C, 32°C) conditions.The reference genome was used for the further transcritome analysis of genes expression profiels in eystalk of Eriocheir sinensis under different tempetures and provide key molecular targets for elucidating the mechanisms by which temperature affects molting and development in E. sinensis.
Project description:transcriptome sequencing was performed to analyze the gene expression profiles of Eriocheir sinensis under normal temperature (22°C) and high temperature (27°C, 32°C) conditions. The differentially expressed genes (DEGs) were identified to provide key molecular targets for elucidating the mechanisms by which temperature affects molting and development in E. sinensis.
Project description:Chinese mitten crab (Eriocheir sinensis) is one of the most popular bred crustaceans in China. E. sinensis stressed by high temperature and resulted in disease and large mortality in summer. Litter is known about the mechanism of temperature tolerant E. sinensis. In this study, transcriptome and proteome for high temperature tolerant (HTT) and high temperature sensitive (HTS) female E. sinensis were identified
2026-02-23 | PXD051770 | Pride
Project description:Hepatopancreas of Eriocheir sinensis
Project description:As representatives of n-6 and n-3 fatty acids, many studies have analyzed the use of soybean oil and linseed oil rich in linoleic acid (18:2n-6, LA) and α-linolenic acid (18:3n-3, LNA) as better substitutes for fish oil. In aquatic animals, different dietary ratios of LA and LNA could have significant effects on growth, lipid metabolism, immune response, and reproduction. To assess the nutritive value of these two fatty acids for the Chinese mitten crab (Eriocheir sinensis), we performed transcriptome analysis and proteomic analysis using label-free quantification of the hepatopancreas of mitten crabs fed with LA or LNA diet. Our results provide new insights for further investigation into the replacement of fish oil from mitten crabs with vegetable oils and enable us to better understand the different roles and nutrition value of LA and LNA in mitten crabs.
2019-08-14 | PXD009864 | Pride
Project description:Transcriptome sequencing of hepatopancreas of Eriocheir sinensis
Project description:We sequenced the mRNA in the Y-organ of normal and eyestalk-ablated Eriocheir Sinensis and obtained the transcriptome profiling (RNA-seq) of the two samples with Next-generation sequencing (NGS) technique. The unigene expression of the two samples were compared and the differetially expressed unigenes were listed.
2020-07-01 | GSE153552 | GEO
Project description:Hepatopancreas of Eriocheir sinensis under starvation conditions
