Project description:Hypertensive disorders in pregnancy, of which the multisystem pathology pre-eclampsia is most severe, often lead to preterm delivery, maternal mortality and life-long complications. Pre-eclampsia lacks early screening tools and causal therapies, illustrating the urgent need for a better understanding of early disease dynamics. Here, we present the first study comparing single-nuclei transcriptomes of human diseased preterm preeclamptic placentae and healthy controls, embedding the characterization of the maternal-fetal barrier dysfunction in the context of a comprehensive spatio-temporal study including early and late gestational placentae. Our results highlight and contextualize a perturbed communication from fetal to maternal side during the development of pre-eclampsia starting with a dysregulated trophoblast stem-cell maturation. We provide new targets for potential early disease prevention in order to protect mother and child from increased gestational mortality and morbidity but also from life-long increased cardiovascular disease risk.
Project description:Oral and oropharyngeal squamous cell carcinoma (OSCC and OPSCC) represents an increasing problem in the global public health. OPSCC is the most frequent malignancy in oral cavity, with an overall increasing incidence and mortality. In view of its unclear exact mechanism and lack of optimized prevention and treatment methods, new biomarkers are needed to characterize the diversity of cancer and to explore effective treatment targets. This study combined translatomics and transcriptomics to analyze the differential genes in human oral cancer and adjacent tissues, in order to provide a new perspective for the prevention and treatment of OPSCC.
Project description:Oral and oropharyngeal squamous cell carcinoma (OSCC and OPSCC) represents an increasing problem in the global public health. OPSCC is the most frequent malignancy in oral cavity, with an overall increasing incidence and mortality. In view of its unclear exact mechanism and lack of optimized prevention and treatment methods, new biomarkers are needed to characterize the diversity of cancer and to explore effective treatment targets. This study combined translatomics and transcriptomics to analyze the differential genes in human oral cancer and adjacent tissues, in order to provide a new perspective for the prevention and treatment of OPSCC.
Project description:Analysis of genome-wide hydroxymethylation within infant placental tissue collected at term. These samples have been collected from the RhodeIsland Child Health Study (RICHS) cohort.
Project description:Analysis of genome-wide hydroxymethylation within infant placenta tissue collected at term. These samples have been collected from the Rhode Island Child Health Study (RICHS) cohort.
Project description:Genome-wide profiling of placental DNA methylation in relation to neurobehavioral development. The Illumina 450k methylation array was used to profile 335 samples. These samples have been collected from the Rhode Island Child Health Study (RICHS). Illumina analysis performed on 335 human placentas
Project description:Analysis of transcript abundance estimates as a function of child soldier status, PTSD symptoms, and psychological resilience. Gene expression profiling was conducted on dried blood spot (DBS) samples collected from community dwelling adolescents and young adults in Nepal. Approximatley half of the sample were former child soldiers in the Nepal People's War and the other half were demographically similiar civilian non-combatants. In addition to basic demographic characteristics (age, sex, ethnic minority status, social caste status, education level), participants were also assessed on syptoms of post-traumatic stress (PTS, assessed by a culturally adapted version of The Child PTSD Symptom Scale; Kohrt BA, et al. (2011) Validation of cross-cultural child mental health and psychosocial research instruments: adapting the Depression Self-Rating Scale and Child PTSD Symptom Scale in Nepal. BMC Psychiatry 11(1):e127, with higher values indicating greater PTSD symptoms) and psychological resilience (assessed by a culturally adapted version of the Resilience Scale; Wagnild GM & Young HM (1993) Development and psychometric evaluation of the Resilience Scale. Journal of Nursing Measurement, with higher values indicating greater resilience). Dichotomous variables were coded 0=no/absent and 1=yes/present. Valid gene expression data are available for 254 samples.
Project description:Analysis of genome-wide hydroxymethylation within infant placental tissue collected at term. These samples have been collected from the RhodeIsland Child Health Study (RICHS) cohort. Bisulfite and oxidative biulfite converted DNA was hybridized to the 450k platform and processed at the University of Minnesota Genomic Core