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ABSTRACT: Lactobacillus strains of Lueyang Black chicken from 2023

PROVIDER: PRJNA1100978 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28705702_1.fastq.gz	Fastqsanger.gz
	SRR28705702_2.fastq.gz	Fastqsanger.gz
	SRR28705703_1.fastq.gz	Fastqsanger.gz
	SRR28705703_2.fastq.gz	Fastqsanger.gz
	SRR28705704_1.fastq.gz	Fastqsanger.gz

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Project description:This work was to study the transcriptome profiles in the skin of chickens with black versus white skin using high-throughput RNA deep-sequencing technology, to investigate the different expression profiles of the genes involved in skin pigmentation, then look for the main differences between black and white skin colors in Lueyang chickens. 16-week-old white and black female Lueyang chickens (5 birds per color) were selected for the sample collection. A piece of skin (8 mm in diameter) from the left back was collected . Total RNA was extracted from the sample using Trizol reagent . Three RNA samples from either the black or white skin samples were pooled following mRNA isolation. The sequencing of the library was performed using an Illumina HiSeq 2000 (LianChuan Sciences, Hangzhou, China). According the result of sequencing, some colored gene expressions were validated using Real time quantitative polymerase chain reaction (qPCR).

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Project description:The genetic foundation of chicken tail feather color is not very well studied to date, though that of body feather color is extensively explored. In the present study, we used a synthetic chicken dwarf line (DW), which was originated from the hybrids between a black tail chicken breed, Rhode Island Red (RIR) and a white tail breed, Dwarf Layer (DL), to understand the genetic rules of the white/black tail color. The DW line still contain the individuals with black or white tails, even if the body feather are predominantly red, after more than ten generation of self-crossing and being selected for the body feather color. We firstly performed four crosses using the DW line chickens including black tail male to female, reciprocal crosses between the black and white, and white male to female to elucidate the inheritance pattern of the white/black tail. We found that (i) the white/black tail feather colors are independent of body feather color and (ii) the phenotype are autosomal simple trait and (iii) the white are dominant to the black in the DW lines. Furtherly, we performed a genome-wide association (GWA) analysis to determine the candidate genomic regions underlying the tail feather color by using black tail chickens from the RIR and DW chickens and white individuals from DW lines.

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Muscle Proteome of Taihe Black-bone Silky Fowl chicken with TMT-based Highresolution Mass Spectrometry

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Project description:The transcriptome of Lueyang black-bone chickens

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Project description:Transcriptional profiling of the jejunum mucosa with 1.5 fold-change reporter genes in comparing control black-boned chickens under normal temperature (NT) conditon with heat-stress treated black-boned chickens under high temperature (HT) condition. Goal was to determine the differentially expressed genes (DEGs) in co-family black-boned chickens exposure to heat stress based on global chicken gene expression.

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