Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1103100 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR28763319_1.fastq.gz | Fastqsanger.gz | |||
| SRR28763319_2.fastq.gz | Fastqsanger.gz | |||
| SRR28763320_1.fastq.gz | Fastqsanger.gz | |||
| SRR28763320_2.fastq.gz | Fastqsanger.gz | |||
| SRR28763321_1.fastq.gz | Fastqsanger.gz |
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Project description:The increasing aquaculture production volumes have caused an escalating demand for alternative protein feed ingredients. Agro-industrial by-products such as sunflower meal are relatively abundant and cheap, but the inclusion levels are limited due to the presence of antinutritional factors and fiber. Solid state fermentation is a processing method with the aim of reducing the content of fiber and antinutritional factors and improving nutritional value in plant protein raw materials. In this study, Atlantic salmon (Salmo salar) at two commercial-like fish farms were fed diets containing 5% non-fermented sunflower meal (as a control diet) and two experimental diets with 5% or 10% fermented sunflower meal. The field trial lasted for eight and 11 months in a coastal and fjord location, respectively with the aim of comparing the effect of fermented diets on growth performance, gut microbiota, distal intestine histology and gene expression of Atlantic salmon under different environmental conditions. The findings revealed that diets with fermented sunflower meal sustained growth performance, improved intestinal health by reducing the prevalence of prominent inflammation and ectopic goblet cells and promoted gut lactic acid bacteria Lactiplantibacillus and Lactobaccilaceae after long-term feeding. Our results suggest that fermented sunflower meal is suitable as a protein source for Atlantic salmon when included at a level of up to 10% in the diet.
2025-04-01 | GSE267194 | GEO
Project description:Fermented sunflower meal in diets for Atlantic salmon
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Project description:Atlantic salmon was fed with diets based on five plant protein sources combined with soyabean saponins. Diets with corn gluten, sunflower, rapeseed and horsebean produced minor effects while combination of saponins with pea protein concentrate caused enteritis and major transcriptome changes in the distal intestine.
2011-12-21 | GSE34578 | GEO
Project description:In this experiment, we used four diets, one based on FM, a challenging diet with 40% soybean meal (SBM), and two diets containing 40% SBM with 5% of Cyberlindnera jadinii yeast exposed to different down-stream processing conditions (heat-inactivated or autolysation). The immunomodulatory effects of the diets were analyzed in the spleen of Atlantic salmon after 37 days of feeding. The results showed that the inclusion of autolysed C. jadinii (ACJ) was able to modulate the response of Atlantic salmon compared to fish fed the SBM-diet, through the activation of biological pathways related to endocytosis, PPRs-signal transduction and transporter activity. Finally, we suggest that the spleen is a good candidate to characterize the immunomodulatory effects of functional ingredients in Atlantic salmon.
2021-08-12 | GSE174262 | GEO
Project description:Single cell proteins, such as Candida utilis, are known to have immunomodulating effects in the distal intestine (DI) of Atlantic salmon, whereas soybean meal (SBM) can cause soybean meal induce enteritis (SBMIE). Inflammatory or immunomodulatory stimuli at the local level in the intestine may alter the plasma protein profile of Atlantic salmon. These changes can be helpful tools in diagnosis for fish diseases and indicators for fish health. The present work aimed to identify local intestinal tissue responses and changes in plasma protein profiles of Atlantic salmon fed C. utilis yeast, SBM, or combined diets. Fish meal (FM) based diet was used as a control diet and the six experimental diets were: FM diet with 200 g/kg C. utilis (FM200CU) and five diets containing 200 g/kg SBM together with 0 (SBM group), 25, 50, 100 or 200 g/kg C. utilis (SBM25CU, SBM50CU, SBM100CU and SBM200CU groups, respectively). Intestine morphology of fish fed FM200CU where not affected whereas SBM group presented changes characteristic of SBMIE. Low inclusion of C. utilis in SBM diet showed a modulation of immune cell populations, but did not alleviate inflammatory symptom.
2020-05-18 | PXD012051 | Pride
Project description:The present study was conducted to investigate the effect of graded levels of black soldier fly larvae (BSFL) (Hermetia illucens) meal and BSFL paste in extruded diets for Atlantic salmon (Salmo salar). A total of 1260 Atlantic salmon with 34 g of mean initial weight were randomly distributed into 21 fiberglass tanks and fed (n=3) with seven extruded isolipidic and isonitrogenous diets for seven weeks. The experimental diets consisted of a positive control diet based on fishmeal, soy protein concentrate, corn gluten, faba bean and fish oil (Control_1); three diets with increased levels of full lipid BSFL meal, substituting 6.25% (6.25_IM), 12.5% (12.5_IM) and 25% (25_IM) of the protein content of Control_1; two diets with increased levels of full lipid BSFL paste, substituting 3.7% (3.7_IP) and 6.7% (6.7_IP); and of protein from Control_1 and a negative a control with 0.84 % of formic acid (Control_2). We investigate the effect of diets on growth performance, mmune response and health.
2021-02-10 | PXD019125 | Pride
Project description:Black soldier fly larvae meal (BSFL) from Hermetia illucens is a promising alternative protein source in diets for farmed fish. The larvae can efficiently convert low-value organic material into high quality protein in a production cycle with low arable land and freshwater inputs. A few recent studies have shown that BSFL is a suitable protein source for Atlantic salmon (Salmo salar) in smaller controlled experiments. However, industry-relevant field trials conducted under large scale near-commercial conditions over a longer period are lacking. In this study, a feeding trial was performed to evaluate the impact of BSFL on growth performance and health of Atlantic salmon during the grow out phase in seawater, in a commercial site in Vestland county, Norway. A total of 320,000 post-smolt Atlantic salmon were distributed into six duplicate sea cages and fed one of three diets (commercial-like control diet and two test diets partially replacing the protein content of the control diet with 4 % and 8 % defatted BSFL meal) for 21 weeks, until a relevant commercial slaughter size of 4.5-5.0 kg was reached. Health parameters were assessed including histology of the distal intestine (DI), in addition to DI microbiota identification (by 16s rRNA-seq) and salmon RNA-seq of DI and head kidney (HK). The results showed that the inclusion of BSFL meal supported growth performance and had no adverse effect on gut health. The beta diversity of the distal intestine microbiota and the relative abundance of families Lactobacillaceae and the chitinolytic Bacillaceae increased in the fish fed the BSFL diets. Additionally, no histopathological changes were attributable to BSFL meal intake. Results from RNA-seq in DI revealed that BSFL inclusion modulates metabolic processes associated with lipids, the response to estrogens, the activity of immune receptors (to chemokines), phagocytosis and extracellular vesicles. Based on these results, black soldier fly larvae meal is a suitable alternative protein ingredient in inclusions of up to at least 8 % for Atlantic salmon under industrial fish farming conditions.
2024-08-16 | GSE241320 | GEO
Project description:This study was performed to investigate assess the impacts of CO and/or CM containing diets on Atlantic salmon hepatic gene expression in order to identify candidate molecular biomarkers of responses to camelina-containing diets. Atlantic salmon were fed diets with complete or partial replacement of FO and/or FM with camelina oil (CO) and/or camelina meal (CM) in a 16-week trial (Control diet: FO; Test diet: 100% FO replacement with CO, with solvent-extracted FM and inclusion of 10% CM (100COSEFM10CM). A 44K microarray experiment identified liver transcripts that responded to 100COSEFM10CM (associated with reduced growth) compared to FO controls at week 16.
2015-05-12 | GSE56784 | GEO
Project description:Atlantic salmon was fed with diets based on five plant protein sources combined with soyabean saponins. Diets with corn gluten, sunflower, rapeseed and horsebean produced minor effects while combination of saponins with pea protein concentrate caused enteritis and major transcriptome changes in the distal intestine. Microarray analyses were perfromed in distant intestine. Individual samples from fish that received saponing were hybridized to pools from salmon fed with the same feeds without saponins.
2011-12-21 | E-GEOD-34578 | biostudies-arrayexpress
Project description:Fish were fed a standard fish meal (FM) diet or a diet with partial replacement of FM with solvent extracted camelina meal (CM) (8%, 16% or 24% CM inclusion) during a 16-week feeding trial. A significant decrease in growth performance was seen in fish fed the CM inclusion diets. A 44k oligonucleotide array experiment was used to identify any differentially expressed transcripts in the distal intestine of the fish fed the 24% CM diet compared to the control. The expression level of these genes was validated using quantitative polymerase chain reaction, which was also used to measure transcript expression in the fish fed the 8% CM and 16% CM diets. Histopathological analysis was used to quantify any physical signs in inflammation in the distal intestine of the Atlantic salmon fed the CM-containing diets.
2015-11-13 | GSE68732 | GEO