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Exogenous RNA surveillance by proton-sensing TRIM25

ABSTRACT: Exogenous RNA surveillance by proton-sensing TRIM25

PROVIDER: PRJNA1103110 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28764200_1.fastq.gz	Fastqsanger.gz
	SRR28764200_2.fastq.gz	Fastqsanger.gz
	SRR28764201_1.fastq.gz	Fastqsanger.gz
	SRR28764201_2.fastq.gz	Fastqsanger.gz
	SRR28764202_1.fastq.gz	Fastqsanger.gz

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Exogenous RNA surveillance by proton-sensing TRIM25

Project description:Exogenous mRNAs require cellular machinery for delivery and translation but also encounter inhibitory factors. To investigate their regulation, we performed genome-wide CRISPR screens with in vitro-transcribed mRNAs in lipid nanoparticles (LNPs). Heparan sulfate proteoglycans and vacuolar ATPase were identified as mediators of LNP uptake and endosomal escape, respectively. TRIM25 emerged as a key suppressor inducing turnover of both linear and circular mRNAs. Endoribonucleases, N4BP1 and KHNYN, along with ZAP, act redundantly in TRIM25-dependent surveillance. TRIM25 specifically targets mRNAs delivered via endosomes, and its RNA affinity increases at acidic pH, suggesting activation by protons released from ruptured endosomes. N1-methylpseudouridine (m1Ψ) modification reduces TRIM25’s RNA binding, helping RNAs evade its suppressive effect. This study comprehensively maps cellular pathways regulating LNP-mRNAs, offering insights into RNA immunity and therapeutics.

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