Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The inflammatory micro-environment induced by targeted CNS radiotherapy is underpined by disruption of DNA methylation [Array]

ABSTRACT: The inflammatory micro-environment induced by targeted CNS radiotherapy is underpined by disruption of DNA methylation [Array]

PROVIDER: PRJNA1103824 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

The inflammatory micro-environment induced by targeted CNS radiotherapy is underpined by disruption of DNA methylation [Array]

Project description:Although targeted radiotherapy (RT) is integral to the increasing survival of cancer patients, it has significant side-effects, the cellular and molecular mechanisms of which are not fully understood. During RT epigenetic changes occur in neoplastic tissue, but few studies have assessed these in non-neoplastic tissue and results are highly variable. Using bulk DNA methylation and RNA sequencing as well as spatial transcriptomics (ST) in a unique cohort of patient tissue samples, we show distinct differences in DNA methylation patterns in irradiated brain tissue, whilst ST characterisation identifies specific micro-environmental niches present after irradiation and highlights neuropeptides that could be propagating neuroinflammation. We also show that in a cerebral organoid (CO) model of early changes in neurons after irradiation there are similar DNA methylation alterations and disruption of the DNA methylation machinery, suggesting that early but persistent epigenetic dysregulation plays a role in neurotoxicity. We provide a link between radiotherapy induced neuroinflammation and disruption of DNA methylation for the first time and suggest possible driving mechanisms for this chronic neuroinflammation.

2025-04-10 | GSE264703 | GEO

The inflammatory micro-environment induced by targeted CNS radiotherapy is underpined by disruption of DNA methylation

2025-05-02 | GSE272334 | GEO

The inflammatory micro-environment induced by targeted CNS radiotherapy is underpined by disruption of DNA methylation

Project description:The inflammatory micro-environment induced by targeted CNS radiotherapy is underpined by disruption of DNA methylation

| PRJNA1136474 | ENA

The inflammatory micro-environment induced by targeted CNS radiotherapy is underpined by disruption of DNA methylation [RNA-seq]

2025-05-02 | GSE265797 | GEO

The inflammatory micro-environment induced by targeted CNS radiotherapy is underpined by disruption of DNA methylation [RNA-seq]

Project description:The inflammatory micro-environment induced by targeted CNS radiotherapy is underpined by disruption of DNA methylation [RNA-seq]

| PRJNA1104220 | ENA

Disruption of DNA methylation underpins the neuroinflammation induced by targeted CNS radiotherapy

2025-05-02 | GSE289218 | GEO

Disruption of DNA methylation underpins the neuroinflammation induced by targeted CNS radiotherapy

Project description:Disruption of DNA methylation underpins the neuroinflammation induced by targeted CNS radiotherapy

| PRJNA1221921 | ENA

Improved Sensitivity in Low-Input Proteomics using Micro-Pillar Array-based Chromatography

Project description:Capitalizing on the massive increase in sample concentrations which are produced by extremely low elution volumes, nano-LC-ESI-MS/MS is currently the most sensitive analytical technology for the comprehensive characterization of complex protein samples. However, despite tremendous technological improvements made in the production and the packing of monodisperse spherical particles for nano-flow HPLC, current state-of-the-art systems still suffer from limits in operation at the maximum potential of the technology. With the recent introduction of the µPAC system, which provides perfectly ordered micro-pillar array based chromatographic support materials, completely new chromatographic concepts for optimization towards the needs of ultra-sensitive proteomics become available. Here we report on a series of benchmarking experiments comparing the performance of a commercially available 50 cm micro-pillar array column to a widely used nano-flow HPLC column for the proteomics analysis of 10 ng tryptic HeLa cell digest. Comparative analysis of LC-MS/MS-data corroborated that micro-pillar array cartridges provide outstanding chromatographic performance and excellent retention time stability, which substantially increase sensitivity in the analysis of low-input proteomics samples, and thus repeatedly yielded almost twice as many unique peptide and unique protein group identifications when compared to conventional nano-flow HPLC columns.

2019-10-23 | PXD014124 | Pride

Host Micro

Project description:Host Micro Metagenome

| PRJNA793758 | ENA

Micro-BC

Project description:Micro-BC Metagenome

| PRJNA831285 | ENA