Project description:ChIP-seq data for three Mucor spp. from the Mucor circinelloides species complex: PS15, PS14, and PS10. ChIP was performed against H3K9me2 and RNA polymerase II (RNAP). PS10 and PS14 include samples from a wild type (WT) and an epimutant strain (E1 and E3, respectively), while PS15 include a WT from each mating type (- and +), two epimutant strains (E1m, E10p) and their progeny (#10 and #34, respectively)
Project description:We sequenced Endogenous short RNAs in Mucor circinelloides fungus grown in standard liquid culture. Short RNAs were profiled in wild type, Dicer-like 1 mutant (dcl1-), Dicer-like 2 mutant (dcl2-) and double Dicer mutant (dcl1-/dcl2-) strains. We identified many loci that produced less short RNAs in the dcl2- strain suggesting that DCL2 is the major protein generating short RNAs in Mucor circinelloides.
Project description:To elucidate the role of epimutation in antifungal resistance of Mucor, epimutations were isolated that confer resistance to 5-fluoroorotic acid (5-FOA). We identified epimutant strains that exhibit resistance to 5-FOA without mutations in either of the 5-FOA target genes, pyrF or pyrG. Using sRNA sequencing, we demonstrate that these epimutants harbor sRNA against either pyrF or pyrG, and further show that this sRNA is lost after reversion to drug sensitivity.
