Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1106273 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR28842318_1.fastq.gz | Fastqsanger.gz | |||
| SRR28842318_2.fastq.gz | Fastqsanger.gz | |||
| SRR28842319_1.fastq.gz | Fastqsanger.gz | |||
| SRR28842319_2.fastq.gz | Fastqsanger.gz | |||
| SRR28842320_1.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 12 |
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Project description:Transcriptome analysis of peritoneal lavage of mice infected with T. gondii Toxoplasma gondii is the causative agent of toxoplasmosis in human and animals. In mouse model, T. gondii strains can be divided into three groups, including the virulent, intermediately virulent and non-virulent. The clonal Type I, II and III T. gondii strains belong to these three groups respectively. To better understand the basis of virulence phenotypes, we investigated mouse gene expression responses to the infection of different T. gondii strains at day 5 post intraperitoneal inoculation with 500 tachyzoites. The transcriptomes of mouse peritoneal cells showed that 1927, 1573, and 1009 transcripts were altered more than 2 fold by Type I, II and III infections, respectively, and majority of altered transcripts were shared. Overall transcription patterns were similar in Type I and Type II infections and both had greater changes than that of Type III. Quantification of parasite burden in mouse spleens showed that Type I was 1000 times higher than Type II, and Type II was 20 times higher than Type III. Fluorescence activated cell sorting revealed that Type I and II infections had comparable macrophage populations and both were higher than Type III infection. In addition, Type I infection had higher percentage of neutrophils than that of Type II and III. Taken together, these results suggested that there is a common gene expression response to T. gondii infection in mice. This response is further modified by parasite strain specific factors that determine their distinct virulence phenotypes. We analyzed mRNA from female CD1 outbred mice, 6-8 weeks old infected with Type I, II and III T. gondii strains. We used the Affymetrix Mouse Gene 1.0 ST platform. Raw array data was processed by Partek® Genomics SuiteTM software. Three replicates were performed for Type I-GT1 and Type III-CTG and two replicates for Type II- PTG.
2011-12-22 | E-GEOD-34416 | biostudies-arrayexpress
Project description:Estrogen induce organ-specific cell proliferation and development in female reproductive organs, though the reproductive differentiation, sex maturation, implantation and lactation. However, the mechanism of organ-specific estrogen responsive genes is unknown. Thus, we examined early estrogen responsive genes in mouse uterus, vagina and mammary gland. Keywords: organ specificity
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Project description:Toxoplasma gondii is a protozoan parasite with a remarkable neurotropic affinity. We recently showed that T. gondii infection can alter the global metabolism of the cerebral cortex of the mice. However, the impact of this infection on the metabolism of the cerebellum remained unclear. In this study, we compared the metabolomic profiles of mouse cerebellum following acute and chronic infection with T. gondii. Three-week-old female BALB/c mice (n = 36) were commercially obtained from Lanzhou University Laboratory Animal Center (Lanzhou, China). Mice were separated into 6 groups (6 mice/group). The mice in the infected groups were challenged with 10 T. gondii cysts of PRU strain suspended in 0.5 ml phosphate-buffered saline (PBS), whereas the mice in the control groups were sham treated with 0.5 ml PBS only. All mice were provided non-medicated feed and water ad libitum during the experiment. The mice were monitored twice daily for signs of illness and mortality. At 7, 14, and 21 days post infection (dpi), mice from infected and control group were humanely sacrificed by CO2 asphyxiation and cerebellum of each mouse was dissected with scissors and forceps. Then, the metabolite profile changes in the cerebellum of mice following infection of T. gondii was analyzed.
2020-04-27 | MTBLS1537 | MetaboLights
Project description:We sequenced the cerebral cortex transcriptome of both male and female mice either infected with T. gondii or age-matched uninfected controls. These transcriptomes were determined along the infection timeline with early (21 and 28 days) and late (3 and 6 months) chronic represented. We obtained 5.7 billion paired-end sequences and aligned them to the Mus musculus genome. We found that a majority of the host genes with greater abundance at day 21 post-infection are still more abundant 6 months post-infection in male and female mice, although, females have far fewer genes that are significantly less abundant. Genes that were significantly less abundant in males are involved with the immune response, and may lead to the slight increase in cyst numbers in males during chronic infection.
2018-07-24 | GSE117504 | GEO
Project description:The goal of this study was to identify the neural systems that underlie the behavorial changes in intermediate hosts of Toxoplasma gondii. Using a mouse whole genome microarray, we analyzed gene expression in the frontal cortex during the latent stages of infection. We found marked sex-dependent effects on gene transcription in mice. In female mice, Toxoplasma infection altered the expression of genes involved in the development of the forebrain, neurogenesis, sensory and motor coordination. In male mice, infection led mainly to modulation of genes associated with olfaction. Our results indicate that the gender of the host plays a major role in determining variable brain changes following Toxoplasma infection. Male and female BALB/c mice (infected female: n=5; infected male: n=5; female control: n=5; male control: n=4) were used in this study. They were sacrificed approximately 6 month post infection and the frontal cortices harvested. RNA transcript levels were quantified by microarray analyses using Illumina Mouse Ref-8 v2.0 Expression BeadChips.
2014-07-28 | E-GEOD-31207 | biostudies-arrayexpress
Project description:Transcriptome analysis of peritoneal lavage of mice infected with T. gondii Toxoplasma gondii is the causative agent of toxoplasmosis in human and animals. In mouse model, T. gondii strains can be divided into three groups, including the virulent, intermediately virulent and non-virulent. The clonal Type I, II and III T. gondii strains belong to these three groups respectively. To better understand the basis of virulence phenotypes, we investigated mouse gene expression responses to the infection of different T. gondii strains at day 5 post intraperitoneal inoculation with 500 tachyzoites. The transcriptomes of mouse peritoneal cells showed that 1927, 1573, and 1009 transcripts were altered more than 2 fold by Type I, II and III infections, respectively, and majority of altered transcripts were shared. Overall transcription patterns were similar in Type I and Type II infections and both had greater changes than that of Type III. Quantification of parasite burden in mouse spleens showed that Type I was 1000 times higher than Type II, and Type II was 20 times higher than Type III. Fluorescence activated cell sorting revealed that Type I and II infections had comparable macrophage populations and both were higher than Type III infection. In addition, Type I infection had higher percentage of neutrophils than that of Type II and III. Taken together, these results suggested that there is a common gene expression response to T. gondii infection in mice. This response is further modified by parasite strain specific factors that determine their distinct virulence phenotypes.
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