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Effect of deletion of NELL2 on gene expression in calvarial preosteoblasts

ABSTRACT: Effect of deletion of NELL2 on gene expression in calvarial preosteoblasts

PROVIDER: PRJNA1106360 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28843907_1.fastq.gz	Fastqsanger.gz
	SRR28843907_2.fastq.gz	Fastqsanger.gz
	SRR28843908_1.fastq.gz	Fastqsanger.gz
	SRR28843908_2.fastq.gz	Fastqsanger.gz
	SRR28843909_1.fastq.gz	Fastqsanger.gz

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Effect of deletion of NELL2 on gene expression in calvarial preosteoblasts

Project description:Neural EGFL-like 2 (NELL2) is a secreted protein known for its regulatory functions in the nervous and reproductive systems, yet its role in bone biology remains unexplored. In this study, we observed that the NELL2 levels were diminished in the serum of osteoporosis patients, as well as in the bone of aged and ovariectomized (OVX) mice. In vitro loss-of-function and gain-of-function studies revealed that NELL2 facilitated osteoblast differentiation and impeded adipocyte differentiation from mesenchymal progenitor cells. In vivo studies further demonstrated that the deletion of NELL2 in preosteoblasts resulted in a low bone turnover phenotype characterized by decreased cancellous bone mass in mice. Mechanistically, we identified Fibronectin 1 (Fn1) as a potential receptor for NELL2. Specifically, NELL2 interacted with the FNI-type domain located at the C-terminus of Fn1. Moreover, we found that NELL2 activated the focal adhesion kinase (FAK)/AKT signaling pathway through Fn1/integrin β1 (ITGB1), leading to the promotion of osteogenesis and the inhibition of adipogenesis. Notably, administration of NELL2-AAV was found to ameliorate bone loss in OVX mice. These findings underscore the significant role of NELL2 in osteoblast differentiation and bone homeostasis, suggesting its potential as a therapeutic target for managing osteoporosis.

2025-04-11 | GSE266198 | GEO

Effect of Fn1 knockdown or NELL2 overexpression on gene expression in stromal ST2 cells

2025-04-11 | GSE265936 | GEO

Parathyroid Hormone Treatment Effect on Gene Expression in Primary Calvarial Osteoblasts

Project description:Parathyroid Hormone Treatment Effect on Gene Expression in Primary Calvarial Osteoblasts

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Effect of miR17-92 and miR-106b-25 loss in primary calvarial cells

Project description:Gene expression profiling of primary calvarial cells with miR-17-92:miR-106b-25 conditional deletion

2019-07-04 | GSE114495 | GEO

Effect of Fn1 knockdown or NELL2 overexpression on gene expression in stromal ST2 cells

Project description:Effect of Fn1 knockdown or NELL2 overexpression on gene expression in stromal ST2 cells

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RNA Sequencing Analysis of Wild Type and Nell2-KO Caput Epididymal Transcriptomes

Project description:Purpose: The goal of this study is to detect differentially expressed genes, between Wild type (WT) and Nell2-/- (Nell2-KO) caput epididymis by RNA sequencing Methods: Caput epidiymal mRNA profiles of 14-week-old wild-type (WT) and NEL-like 2 knockout (Nell2-KO) mice were generated by deep sequencing, in quadplicate, using Illumina GAIIx. Results: RNA-seq data identified differentially expressed transcripts; 26 genes showed differential transcript expression between the WT and Nell2-KO caput epididymis, with WT RPKM ≥100, a fold change ≤0.1, and t-test p value <0.05. Altered expression of 26 genes identified. Conclusions: Our results show that the expression of many genes were downregulated in Nell2-KO caput epididymis compared with WT one.

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Neural Epidermal Growth Factor-Like Like Protein 2 (NELL2) is Expressed in Human Oligodendroglial Cell types

Project description:NELL2 is a cytoplasmic and secreted glycosylated protein with six epidermal growth factor-like domains. In animal models, Nell2 is predominantly expressed in neural tissues where it regulates neuronal differentiation, polarization, and axon guidance, but little is known about the role of NELL2 in human brain development. In this study, we show that rostral neural stem cells (rNSC) derived from human iPSC exhibit particularly strong NELL2 expression, and that NELL2 protein is enriched at the apical side of neural rosettes in hiPSC derived brain organoids. Following differentiation of human rostral NSC into neurons, NELL2 remains robustly expressed but changes its subcellular localization from >20 small cytoplasmic foci in NSC to 1-5 large peri-nuclear puncta per neuron. Unexpectedly, we discovered that in human brain organoids NELL2 is readily detectable in oligodendroglia and that the number of NELL2 puncta increases as oligodendrocytes mature. Artificial intelligence-based machine learning further predicts a strong association of NELL2 with multiple human white matter diseases, suggesting that NELL2 may possess yet unexplored roles in regulating oligodendrogenesis and/or myelination during human cortical development and maturation.

2021-12-13 | GSE190633 | GEO

HSALNG0134318 deletion effect on protein expression

Project description:We generated HK2 cell lines with deletion of the locus HSALNG0134318 locating in human 22q11.2, which was predicted to be associated with congenital anomalies of the kidney and urinary tract (CAKUT) . Proteomic changes was assessed to investigate the deletion effect of HSALNG0134318

2025-01-01 | PXD059368 |

effect of slc11a2 deletion on placental gene expression

Project description:effect of slc11a2 deletion on placental gene expression

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Expression data from mouse calvarial suture mesenchymal cells

Project description:Axin2-expressing calvarial suture stem cells can contribute to calvarial development, homeostatic maintenance, repair, and regeneration. We used microarray to examine the gene expression profiles of Axin2-expressing suture stem cells and Axin2-negative cells in suture mesenchyme.

2015-11-11 | GSE74849 | GEO

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