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Genes co-regulated by p85beta and BCLAF1 in ovarian cancer cells

ABSTRACT: Genes co-regulated by p85beta and BCLAF1 in ovarian cancer cells

PROVIDER: PRJNA1107399 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28891944_1.fastq.gz	Fastqsanger.gz
	SRR28891944_2.fastq.gz	Fastqsanger.gz
	SRR28891945_1.fastq.gz	Fastqsanger.gz
	SRR28891945_2.fastq.gz	Fastqsanger.gz
	SRR28891946_1.fastq.gz	Fastqsanger.gz

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Genes co-regulated by p85beta and BCLAF1 in ovarian cancer cells

Project description:p85beta of the PI3K can localize to the nucleus. Our data showed that it can increase the expression of a transcription factor BCLAF1. To determine whether p85beta has any affect on gene transcription and to identify gene targets that are regulated through BCLAF1, RNAseq was performed on p85beta-expressing cells transfected with BCLAF1 siRNA .

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Expression data after BCLAF1-L knockdown in human RKO cells

Project description:Bcl-2-accociated transcription factor 1(BCLAF1) has been reported to be involved in diverse biological processes. Alternative splicing leads to mutiple transcript virants in human cells and we are interested in functions of its full length isoform(BCLAF1-L) in human colon cancer cells, thus to understanding its role in colon cancer progression. We used microarray to detail the global programme of gene expression after BCLAF1-L knockdown(sh-BCLAF1-L#1) in RKO cells and identified distinct classes of up-regulated or down-regulated genes impaired by its inhibition, when comparing with the control group(sh-Luci). RKO cells were infected with retroviruses either expressing control (sh-Luci) or BCLAF1-L knockdown (sh-BCLAF1-L#1). Medium was replaced 24h after infection, and infected cells were selected by the addition of puromycin (2ug/ml) for 72-96h. Then cells were havested for RNA extraction and hybridization on Affymetrix microarrays.

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Identification of p85beta- or BCLAF1-enriched genomic regions

Project description:p85beta of the PI3K can localize to the nucleus. Our immunoprecipitation data showed that it physically interacts with a transcription factor BCLAF1. To reveal the enrichment of p85beta and BCLAF1 at genomic regions and to determine whether p85beta and BCLAF1 co-occupy at genomic regions, chromatin immunoprecipitation sequencing (ChIP-seq) was performed.

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Expression data after BCLAF1-L knockdown in human RKO cells

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Genes regulated by miR-328-3p in ovarian cancer cells

Project description:Genes regulated by miR-328-3p in ovarian cancer cells

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BCLAF1 Chip-chip control (siGFP) and BRCA1 depleted (siBRCA1) 293T cells in the absense or presense of Etoposide

Project description:BCLAF1 is a serine-arginine (SR) protein implicated in transcriptional regulation and mRNA splicing. We have recently identified BCLAF1 as part of a novel mRNA splicing complex that is recruited to different genetic promoters by the breast cancer susceptiblity protein, BRCA1 in response to DNA damage. This ChIP-chip study was designed to identify genes/promoters regulated by the BRCA1/BCLAG1 mRNA splicing complex by identifying promoters bound by BCLAF1 in the absense and presense of BRCA1 in control cells and cells treated with etoposide to induce DNA damage. This study includes tripicate BCLAF1 ChIP-chip experiments in untreated and etoposide treated (1uM 16 hours) control cells (siGFP) and cells depleted of BRCA1 (siBRCA1). Chromatin Immunoprecipitaitons were performed in triplicate with BCLAF1 antibodies in control 293T cells transfected with siGFP siRNAs and BRCA1 siRNAs (siBRCA1 to deplete BRCA1). Immunoprecipitated genomic DNA was labelled with Cy3 and Input genomic DNA was labelled with Cy5 and hybridized to NimbleGen human 3x720k RefSeq promoter arrays to identify BCLAF1 boundgenomic DNA regions.

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2021-09-24 | PXD027611 | Pride

Genes regulated by Hfq

Project description:Genes regulated by Hfq

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