Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Wars1 loss-of-function in the liver identifies a unique cytosol-to-mitochondria stress response.

ABSTRACT: Wars1 loss-of-function in the liver identifies a unique cytosol-to-mitochondria stress response.

PROVIDER: PRJNA1107534 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28887000_1.fastq.gz	Fastqsanger.gz
	SRR28887000_2.fastq.gz	Fastqsanger.gz
	SRR28887001_1.fastq.gz	Fastqsanger.gz
	SRR28887001_2.fastq.gz	Fastqsanger.gz
	SRR28887002_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 48

Similar Datasets

Wars1 loss-of-function in the liver identifies a unique cytosol-to-mitochondria stress response.

Project description:Several laboratories, including ours, have used the Slc25a47tm1c(EUCOMM)Hmgu mouse model to characterize the role of SLC25A47, an hepatocyte-specific mitochondrial carrier, in hepatic metabolism and systemic physiology. In this study, we report that the hepatic and systemic phenotypes observed in Slc25a47tm1c(EUCOMM)Hmgu mouse model upon recombination of the Slc25a47-Wars1 locus, are driven by the unanticipated downregulation of Wars1, the cytosolic tryptophan aminoacyl-tRNA synthetase whose gene is located in close vicinity to Slc25a47. We show that downregulation of Wars1 in hepatocytes not only impacts on cytosolic translation, as expected, but also impairs the ability of hepatic mitochondria to translate proteins eventually leading to a profound activation of the mitochondrial unfolded protein response (UPRmt), an integral part of the mitochondrial stress response (MSR). Our results clarify the respective roles of Slc25a47 and Wars1 in both systemic and hepatic metabolism and suggest that a bidirectional stress communication system operates in the mammalian liver between the cytosol and mitochondria highlighting the complexity and promiscuity of cellular stress mechanisms. This newly identified anterograde pathway complements the previously recognized Mitochondrial-to-Cytosolic Stress Response (MCSR), which we propose to call the Cytosolic-to-Mitochondrial Stress Response (CMSR).

2025-05-01 | GSE266539 | GEO

Proteomic analysis of liver cytosol and mitochondria of apoE-KO mice treated with TUG-891.

Project description:ApoE-KO mice were treated with FFAR4 agonist TUG-891 for 16 weeks. Protein abundance changes in relation to untreated control were measured in liver samples fractionated to cytosol and mitochondria by SWATH-MS.

2024-08-09 | PXD028535 | Pride

Acute detachment of hexokinase II from mitochondria modestly increases oxygen consumption of the intact mouse heart

Project description:Cardiac hexokinase II (HKII) can translocate between cytosol and mitochondria and change its cellular expression with pathologies such as ischemia–reperfusion, diabetes and heart failure. The cardiac metabolic consequences of these changes are unknown. Here we measured energy substrate utilization in cytosol and mitochondria using stabile isotopes and oxygen consumption of the intact perfused heart for 1) an acute decrease in mitochondrial HKII (mtHKII), and 2) a chronic decrease in total cellular HKII.

2021-04-27 | GSE173341 | GEO

Comparative functional genomics identifies unique molecular features of EPSCs

Project description:Comparative functional genomics identifies unique molecular features of EPSCs

| PRJNA830833 | ENA

The RNA seq of cytomatrix and cytosol

Project description:The RNA seq of cytomatrix and cytosol

| PRJNA820634 | ENA

Single cell RNA sequencing identifies unique inflammatory airspace macrophage subsets

Project description:Single cell RNA sequencing identifies unique inflammatory airspace macrophage subsets

| PRJNA491204 | ENA

ENCODE: PolyA+ RNA from cytosol of HeLa and GM06990 cells (NCBIv35)

Project description:PolyA+ RNA from cytosol of HeLa and GM06990 cells using Affymetrix ENCODE tiling chip with NCBI build 35 annotation. PolyA+ RNA from cytosol of HeLa and GM06990 cells.

2010-06-05 | E-GEOD-3663 | biostudies-arrayexpress

Micro RNA expression in human skeletal muscle mitochondria

Project description:MiRNAs are non-coding RNAs that regulate gene expression. MiRNAs mostly localise within the cytosol but are also found in the mitochondria where they can regulate the expression of mitochondrial-encoded transcripts. Aberrant miRNA expression is linked to mitochondrial dysfunction in chronic metabolic conditions. However, the role of miRNAs in the regulation of mitochondrial function in healthy tissue, such as in response to exercise, is not known. The aim of this study was to use a small RNA library preparation approach to profile the entire population of miRNAs in human skeletal muscle mitochondria and investigate if mitochondrial miRNA expression was altered following an acute endurance exercise.

2026-04-13 | GSE220280 | GEO

Micro RNA expression in rodent skeletal muscle mitochondria

Project description:MiRNAs are non-coding RNAs that regulate gene expression. MiRNAs mostly localise within the cytosol but are also found in the mitochondria where they can regulate the expression of mitochondrial-encoded transcripts. Aberrant miRNA expression is linked to mitochondrial dysfunction in chronic metabolic conditions. However, the role of miRNAs in the regulation of mitochondrial function in healthy tissue, such as in response to endurance exercise training, is not known. The aim of this study was to use a small RNA library preparation approach to profile the entire population of miRNAs in rat skeletal muscle mitochondria and investigate if mitochondrial miRNA expression was altered following4 weeks of endurance training

2025-07-02 | GSE220557 | GEO

Mitochondrial depolarization stabilizes the vitamin B12 chaperone MMADHC in the cytosol to increase MTR activity

Project description:Of the ~1100 mitochondrial proteins, only a handful like PINK1 and ATFS-1 are known to stabilize and relocalize upon collapse of the proton motive force (PMF) to execute signaling roles. To systematically identify genes that increase exclusively at the protein level upon PMF collapse, we performed a joint proteomic and RNA-seq screen. The screen revealed 10 candidates (six mitochondrial), including the vitamin B12 chaperone MMADHC and cytosolic B12-dependent 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR). MMADHC is short-lived across cell types and we show that its levels increase with PMF collapse. MMADHC stabilization precedes PINK1 activation in a time course of increasing mtDNA depletion, suggesting greater sensitivity to PMF collapse. MMADHC accumulates in mitochondria with LONP1 inhibition but in the cytosol upon PMF collapse, likely due to mitochondrial import failure. Cytosol-stabilized MMADHC increases MTR levels and activity. Altogether, the mitochondrial PMF regulates the cytosolic B12-dependent MTR, integral to one-carbon metabolism, by controlling the stability and compartmentalization of the B12 chaperone MMADHC.

2026-08-21 | GSE335487 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data