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ABSTRACT: The different leaf positions of Longjing43 tea plant

PROVIDER: PRJNA1110611 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR29001084_1.fastq.gz	Fastqsanger.gz
	SRR29001084_2.fastq.gz	Fastqsanger.gz
	SRR29001085_1.fastq.gz	Fastqsanger.gz
	SRR29001085_2.fastq.gz	Fastqsanger.gz

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Project description:The different leaf positions of Shuchazao tea plant

| PRJNA563742 | ENA

Impact of exogenous caffeine on regulatory networks of microRNAs in response to Colletotrichum gloeosporioides in tea plant.

Project description:Anthracnose, caused by Colletotrichum gloeosporioides is one of the most serious diseases of tea plant [Camellia sinensis (L.) O. Kuntze]. MicroRNAs are key modulators of gene expression in defense responses and plant immunity; although, foliar application of exogenous caffeine in anthracnose disease control management has proven to be effective, miRNA-mediated regulatory mechanisms underlying caffeine-induced plant defense response to C. gloeosporioides remain unexplored in tea plant. Using high-throughput-sequencing, 24 miRNA sequencing data sets and 8 degradome data sets were generated from the susceptible cultivar Longjing43 (LJ43) and the resistant cultivar Zhongcha108 (ZC108) leaves treated with CK (Water), C. gloeosporioides-inoculation (CgI), exogenous caffeine (CN) and CgI + CN. Using sRNA sequencing, 424 conserved miRNAs and 417 novel miRNAs were identified; of these, 146 and 130 miRNAs were differentially expressed under CgI + CN treatment in the LJ43 and ZC108, respectively. Degradome sequencing identified 599 targets predicted to be cleaved by 210 conserved and 70 novel miRNAs. Majority of the annotated targets were found to involve in regulation of transcription factors, oxidation-reduction and metabolic process for plant growth and development as well as stress responses in tea plant against C. gloeosporioides stress. The expression pattern of eight miRNAs and their targets were validated by qRT-PCR, and correlation analysis of csn-miR164a_R+1_1ss21AG/NAC-17 and csn-miR396b-5p/GRF-1 showed highly significant negative R-value at 7th dpi under CgI + CN in the LJ43. This study provides important insights into the novel approach of exogenous caffeine-induced miRNAs dynamically exerts its fungicidal activity through regulating JA/ET signaling pathway, thereby accurately switch on LJ43 susceptibility nature to resistance activity against C. gloeosporioides infection.

2021-12-29 | GSE146713 | GEO

Camellia sinensis cultivar:Shuchazao

Project description:tea plant leaf Transcriptome

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Xinyang Maojian green tea (Camellia sinensis) microRNAs data in different leaf development stages.

Project description:The microRNAs reveal the molecular mechanism involved in the regulation of leaf development in Xinyang Maojian green tea.

2023-03-05 | GSE224544 | GEO

Expression profiling changes during different albescent stages of 'Anji Baicha' (Camellia sinensis)

Project description:The RNA-Seq was used to analyze the expression profiling of genes in different ablescent stages of 'Anji Baicha' Examination of three tea leaf samples in yellow stage, white stage and green stage

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Next generation sequencing analysis of tea plant transcriptomes in response to Colletotrichum camelliae infection.

Project description:Next generation sequencing (NGS) was performed to identify genes changed in tea plant upon Colletotrichum camelliae infection. The goal of the work is to find interesting genes involved in tea plant in response to fungi infection. The object is to reveal the molecular mechanism of tea plant defense.

2024-07-18 | GSE208559 | GEO

Next-generation sequencing analysis of tea plant cultivar transcriptomes in response to Colletotrichum camelliae infection

Project description:Next-generation sequencing (NGS) was performed to identify genes changed in tea plant cultivar Zhongcha 108 upon Colletotrichum camelliae infection. The goal of the work is to find interesting genes involved in tea plant in response to fungi infection. The object is to reveal the molecular mechanism of tea plant defense.

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Transcriptional landscape of Camellia sinensis roots at single- nucleus resolution

Project description:In summary, this study identifies most cell types in the root tips of the tea plant through single-nucleus transcriptomic analysis, establishing marker genes and cell type-specific regulatory programs. The evolutionary conservation and divergence in cell type and species-specific gene expression between tea plant and Arabidopsis provide new insights into the functions and evolution of cell types in tea plant. And it provides a detailed transcriptomic overview of the main cell types at the root tips of the tea plant at single-nucleus resolution.

2025-08-01 | GSE273722 | GEO

Genome-wide identification of microRNAs responsive to Ectropis oblique feeding in the tea plant (Camellia sinensis L.)

Project description:micro RNA identification of tea plant

2017-10-31 | GSE98474 | GEO

Metabolomics reveals the importance of metabolites in Mussaenda pubescens for antioxidant properties and quality traits

Project description:Mussaenda pubescens (Mp) is a valuable medicinal plant that has traditionally been used for medicinal purposes or as a tea substitute. However, there are few studies on the comprehensive and dynamic evaluation of Mp metabolites presented in Mp. This study used an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach and biochemical analysis to investigate substance changes in leaves at three different stages and elucidate the relationship between metabolites and antioxidant capacity. The findings showed that MpP leaves contained 957 metabolites, the majority of which were phenolic acids, lipids, and terpenoids. The metabolite profiling of Mp leaves was significantly influenced by their growth and development at different stages. A total of 317 differentially accumulated metabolites (DAMs) were screened, including 150 primary metabolites and 167 secondary metabolites, with 202 DAMs found in bud leaf vs. tender leaf, 54 DAMs in tender leaf vs. mature leaf, and 254 DAMs in bud leaf vs. mature leaf. Total phenolics, flavonoids, and anthocyanin concentrations decreased as Mp leaves grew and developed, whereas terpenoids increased significantly. The secondary metabolites also demonstrated a positive correlation with antioxidant activity. Phenolics, flavonoids, terpenoids, and anthocyanins were the primary factors influencing the antioxidant activity of leaves. These findings provide new insights into the metabolite formation mechanism, as well as the development and utilization of Mp tea.

2024-04-08 | MSV000094491 | MassIVE

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