Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile at single cell level of CD4 T cells from the spleens of wildtype (WT) and Interferon regulatory factor 5 (IRF5) knockout (KO) C57BL6/J mice following 6 hour anti-CD3/anti-CD28 stimulation

ABSTRACT: Gene expression profile at single cell level of CD4 T cells from the spleens of wildtype (WT) and Interferon regulatory factor 5 (IRF5) knockout (KO) C57BL6/J mice following 6 hour anti-CD3/anti-CD28 stimulation

PROVIDER: PRJNA1110778 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR29004088_1.fastq.gz	Fastqsanger.gz
	SRR29004088_2.fastq.gz	Fastqsanger.gz
	SRR29004089_1.fastq.gz	Fastqsanger.gz
	SRR29004089_2.fastq.gz	Fastqsanger.gz
	SRR29004090_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Gene expression profile at single cell level of CD4 T cells from the spleens of wildtype (WT) and Interferon regulatory factor 5 (IRF5) knockout (KO) C57BL6/J mice following 6 hour anti-CD3/anti-CD28 stimulation

Project description:The precise mechanisms by which IRF5 regulates CD4 T cell support of the adaptive immune response is still unknown. Here, we used scRNAseq to analyse the impact of loss of Irf5 expression on CD4 T cells following T cell receptor (TCR) stimulation.

2024-09-18 | GSE267271 | GEO

CD28 region-capture HiC of resting and anti-CD3/CD28-stimuated Jurkat cells

Project description:CD28 region-capture HiC of resting and anti-CD3/CD28-stimuated Jurkat cells

| PRJNA1162471 | ENA

Expression data from human peripheral blood lymphocytes stimulated with anti-CD3 and anti-CD28 antibodies.

2013-02-25 | GSE29624 | GEO

Expression data from human peripheral blood lymphocytes stimulated with anti-CD3 and anti-CD28 antibodies.

Project description:Peripheral blood lymphocytes were separated in the Ficoll gradient and subjected for stimulation with anti-CD3 and anti-CD28 antiobodies upon time (6h, 12h and 18h). Next, total RNA was isolated and trenscriptional analysis of stimulated cells was performed. 11 total samples were analysed. Analysis was performed using samples from two healthy donors and one sample from patient. Unstimulated samples were used as a controls.

2013-02-25 | E-GEOD-29624 | biostudies-arrayexpress

ChIP-seq of resting and anti-CD3/CD28-stimuated Jurkat cells

Project description:To finely dissect the dynamic changes in the epigenome and chromatin state during T cell activation, we utilized the Jurkat cell line as a model and performed ChIP-seq on both resting and anti-CD3/CD28-stimulated Jurkat cells, targeting various histone modifications (H3K27ac, H3K4me1, H3K4me3, H3K27me3), RNA PolII, and CTCF.

2025-09-01 | GSE277460 | GEO

ATAC-seq of resting and anti-CD3/CD28-stimuated Jurkat cells

Project description:The majority of genetic risk variants associated with autoimmune diseases are located in chromatin regions that are accessible during T cell stimulation. To systematically investigate the dynamic changes in chromatin accessibility during T cell activation, we utilized the Jurkat cell line as a model and performed ATAC-seq on both resting and anti-CD3/CD28-stimulated Jurkat cells.

2025-09-01 | GSE276690 | GEO

Transcription profiling of human peripheral blood monocytes from a patient with a heterozygous deletion of the CARMA1 gene cultured with anti-CD3, anti-CD3 and anti-CD28 or lipopolysaccharide

Project description:PBMCs were isolated from whole blood taken from one patient and one healthy control. Cells were cultured in media (RPMI) alone, with 1µg/ml anti-CD3, with 1µg/ml anti-CD3 & 1µg/ml anti-CD28 or with 0.5ng/ml LPS. Cells were harvested after 24 or 48 hours, RNA isolated using Trizol, purified using RNeasy columns and labelled using Illumina TotalPrep RNA Amplificiation kit (Applied Biosystems) before hybridisation to Illumina HumanRef-8 arrays.

2022-04-01 | E-MTAB-101 | biostudies-arrayexpress

RNA-seq profiling of in vitro FRC-conditioned, IL6-conditioned or control (anti-CD3/CD28)-stimulated CD8+ T cells

Project description:To broadly assess which pathways FRCs regulate in CD8+ T cells, we sorted CD8+ T cells for RNA-seq following activation in whole splenocyte mixtures via soluble anti-CD3/CD28 for 48 hours (Stim), activated with anti-CD3/CD28 in the presence of Nos2–/– FRCs (FRC) or activated with anti-CD3/CD28 plus 100 ng/ml recombinant IL-6 (IL-6). Here we demonstrate that FRC-derived signals, including IL-6, act in concert with TCR signaling and co-stimulation to promote the expression of MYC and HIF-1-dependent glycolytic genes and vital pro-survival genes (encoding BCL-2 family members, inhibitors of apoptosis [IAPs] members and Cflar) in activated CD8+ T cells.

2019-10-14 | GSE136898 | GEO

ATAC-seq of resting and anti-CD3/CD28-stimuated Jurkat cells

Project description:ATAC-seq of resting and anti-CD3/CD28-stimuated Jurkat cells

| PRJNA1158708 | ENA

RNA-seq of resting and anti-CD3/CD28-stimuated Jurkat cells

Project description:RNA-seq of resting and anti-CD3/CD28-stimuated Jurkat cells

| PRJNA1158706 | ENA