SRNA-seq of Aspergillus fumigatus RNAi double knockouts
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1111541 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR29024400.fastq.gz | Fastqsanger.gz | |||
| SRR29024401.fastq.gz | Fastqsanger.gz | |||
| SRR29024402.fastq.gz | Fastqsanger.gz | |||
| SRR29024403.fastq.gz | Fastqsanger.gz | |||
| SRR29024404.fastq.gz | Fastqsanger.gz |
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Project description:The RNA interference (RNAi) pathway has evolved numerous functionalities in eukaryotes, with many on display in Kingdom Fungi. RNAi can regulate gene expression, facilitate drug resistance, or even be altogether lost to improve virulence potential in some fungal pathogens. In the WHO fungal priority pathogen, Aspergillus fumigatus, the RNAi system is known to be intact and functional. To extend our limited understanding of A. fumigatus RNAi, we performed a multi-condition mRNA-seq analysis comparing expression of several RNAi double knockout mutants with the wild-type strain in conidia and mycelium grown for 24 or 48 hours. The analysis linked the A. fumigatus dicer-like enzymes and RNA-dependent RNA polymerases to regulation of conidial ribosome biogenesis. Cumulatively, A. fumigatus RNAi appears to play an active role in defense against double-stranded RNA species alongside a previously unappreciated housekeeping function in regulation of conidial ribosomal biogenesis genes.
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Project description:sRNA-seq of Aspergillus fumigatus RNAi double knockouts expressing a pksP inverted-repeat transgene
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Project description:The RNA interference (RNAi) pathway has evolved numerous functionalities in eukaryotes, with many on display in Kingdom Fungi. RNAi can regulate gene expression, facilitate drug resistance, or even be altogether lost to improve virulence potential in some fungal pathogens. In the WHO fungal priority pathogen, Aspergillus fumigatus, the RNAi system is known to be intact and functional. To extend our limited understanding of A. fumigatus RNAi, we performed a sRNA-seq to assess sRNAs produced in several RNAi knockouts and a wild type strain expressing a 1200 nt inverted-repeat transgene with complementarity to the pksP gene. We also performed the experiment with a wild-type expressing an IRT with complementarity to pabA. The transgenes harbor a 500-bp inverted repeat. We included an uninduced and induced wild type strain with no inverted-repeat transgene as a control. All conditions were performed in mycelium grown for 24 hours in liquid culture.
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