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ABSTRACT: Polyhydroxyalkanoates production by mixed methanotrophic culture

PROVIDER: PRJNA1111824 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29036801_1.fastq.gz	Fastqsanger.gz
	SRR29036802_1.fastq.gz	Fastqsanger.gz
	SRR29036803_1.fastq.gz	Fastqsanger.gz
	SRR29036804_1.fastq.gz	Fastqsanger.gz
	SRR29036805_1.fastq.gz	Fastqsanger.gz

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Project description:Enrichment of mixed methanotrophic cultures producing polyhydroxyalkanoates (PHAs)

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Proteomic investigation of a mixed microbial culture synthesizing polyhydroxyalkanoates from fermented dairy manure under aerobic feast-famine conditions

Project description:Polyhydroxyalkanoates (PHAs) are bio-based, biodegradable polyesters that can be produced from organic-rich waste streams using mixed microbial cultures. To maximize PHA production, mixed microbial cultures may be enriched for PHA-producing bacteria with a high storage capacity through the imposition of cyclic, aerobic feast-famine conditions in a sequencing batch reactor (SBR). Though enrichment SBRs have been extensively investigated a bulk solutions-level, little evidence at the proteome level is available to describe the observed SBR behavior to guide future SBR optimization strategies. As such, the purpose of this investigation was to characterize proteome dynamics of a mixed microbial culture in an SBR operated under aerobic feast-famine conditions using fermented dairy manure as the feedstock for PHA production. At the beginning of the SBR cycle, excess PHA precursors were provided to the mixed microbial culture (i.e., feast), after which followed a long duration devoid of exogenous substrate (i.e., famine). Two-dimensional electrophoresis was used to separate protein mixtures during a complete SBR cycle, and proteins of interest were identified.

2017-01-31 | PXD003126 | Pride

Proteomic analysis of natural photoheterotrophic mixed consortium for biohydrogen production under nongrowing conditions

Project description:A biohydrogen and polyhydroxyalkanoates(PHA)-producing natural photoheterotrophic mixed culture composed mainly by Rhodopseudomonas palustris and Clostridium sp was studied by a proteomic analysis under non-growth conditions (nitrogen-absence and organic acids). Proteins in C. pasteurianum were upregulated, particularly those related to stress response. In contrast, C. pasteurianum in the consortium did not present such proteins, showing the advantage of being part of it. Both cultures showed proteins involved in organic acid metabolism and biohydrogen production, such as lactate dehydrogenase, ferredoxins, and hydrogenases. Proteomes of R. palustris as single culture and in consortium showed that organic acids were redirect into central carbon pathways to generate reduced equivalents for biohydrogen production. Light-harvesting proteins and fatty acid metabolism linked to PHA accumulation were also upregulated. This study provides insights into how the proteomes of individual organisms and their consortium counterparts adapt to non-growth conditions, shedding light on how microbial interactions influence protein expression.

2025-02-06 | PXD057021 | Pride

Project description:Multi-stage Process for Mixed Microbial Culture Production of Polyhydroxyalkanoates from Sugarcane Stillage: Assessment of External Nutrient Supplementation

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Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [16S]

Project description:Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [16S]

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Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [18S]

Project description:Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [18S]

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Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [ITS]

Project description:Enhanced polyhydroxyalkanoates production from Mixed Microbial Culture through Short Settling Aerobic Dynamic Feeding: improved selection and bulking resilience [ITS]

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metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions

Project description:Purple phototrophic bacteria (PPB) naturally accept CO2 into their metabolism as a primary redox sink system in photo-heterotrophy. Dedicated use of this feature for developing sustainable processes (e.g., through negative-emissions photo-bioelectrosynthesis) requires a deep knowledge of the inherent metabolic mechanisms. Here we provide evidence of the tuning of the PPB metabolic mechanisms upon redox stressing through negative polarization (-0.4 and -0.8 V vs. Ag/AgCl) in photo-bioelectrochemical devices. Using metaproteomic analysis at both reactor ans species level, we showed that a mixed PPB-culture up-regulates its ability to capture CO2 from organics oxidation through the Calvin-Besson-Bassam cycle and anaplerotic pathways, and the redox imbalance is promoted to polyhydroxyalkanoates production. The ecological relationship of PPB with mutualist bacteria stabilizes the system and opens the door for future development of photo-bioelectrochemical devices focused on CO2 up-cycling.

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