Project description:To investigate the intestinal epithelial cellular responses to interferon-gamma (IFNγ), bulk RNA sequencing (RNA-seq) was performed on human small intestinal organoids (enteroids, n = 10) with and without IFNγ treatment (100 pg/ml). The enteroids were derived from endoscopic biopsy samples obtained from patients with Crohn's disease (CD).
Project description:To investigate the intestinal epithelial cellular responses to interferon-gamma (IFNγ), bulk RNA sequencing (RNA-seq) was performed on human small intestinal organoids (enteroids, n = 6) with and without IFNγ treatment (100 pg/ml). The enteroids were derived from endoscopic biopsy samples obtained from patients without inflammatory bowel disease (IBD).
Project description:miRNA expression profiling between GIST and leiomyoma specimens taken by Tunneling Bloc Biopsy Nine GIST patients and seven gastric leiomyoma patients underwent endoscopic biopsy called Tunnel Block Biopsy. MiRNAs were extracted from the tissues, then.
Project description:This study aims to identify and characterize miRNA expression inATLOs isolated by laser microdissection from human AAA biopsy samples. The aim of this study was to profile (with microarray technology) miRNAs in ATLOs (Adventitial tertiary lymphoid organs), isolated by laser capture microdissection (LCM). Smooth muscle cells (SMCs) isolated from control non-aneurysmal aortas were the control group according to which data were normalized. ATLOs were microdissected (mean 13.5mm2) from two different biopsy samples of human abdominal aortic aneurysm. An area enriched in smooth muscle cells was microdissected from two biopsy samples of non aneurysmal abdominal aortas. Each microdissected samples were analyzed independently on microarray.
