Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1113372 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR29087400.fastq.gz | Fastqsanger.gz | |||
| SRR29087401.fastq.gz | Fastqsanger.gz | |||
| SRR29087402.fastq.gz | Fastqsanger.gz | |||
| SRR29087403.fastq.gz | Fastqsanger.gz | |||
| SRR29087404.fastq.gz | Fastqsanger.gz |
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Project description:In planktonic and biofilm mimicking environments, the staphyloccocal transcriptome in t111+t13595 co-cultures showed significant upregulation of genes related to virulence factors contrary to those co-cultures with B. thuringiesis and K. oxytoca. In the biofilm polymicrobial environment, S. aureus transcriptome shows extensive downregulation of gene expression. The animal model co-infection with S. aureus and K. oxytoca proved to be less virulent than when infected only with S. aureus alone, or K. oxytoca alone where higher infection and mortality rates were observed.
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Project description:Bacterial vaginosis (BV) is the most common cause of vaginal discharge among women worldwide. BV is characterized by an imbalance in the vaginal microbiota with depletion of protective Lactobacillus species and overgrowth of facultative and strictly anaerobic bacteria. Although the development of a polymicrobial biofilm on the vaginal epithelium is a hallmark of BV, interactions between key BV-associated bacteria (BVAB) [i.e. Gardnerella vaginalis, Fannyhessea vaginae, and Prevotella bivia] present in the biofilm are still not completely understood. In this study, we aimed to analyse the transcriptome of single and triple-species biofilms growing in the rich medium, New York City III (NYCIII). A previous analysis of triple-species biofilms composition by qPCR showed that the biofilms were mainly composed of G. vaginalis, followed by F. vaginae and P. bivia. The transcriptomic analysis revealed a total of 431 (34 upregulated and 397 downregulated), 126 (36 upregulated and 90 downregulated), and 39 (31 upregulated and 8 downregulated) differentially expressed genes for G. vaginalis, F. vaginae, and P. bivia, respectively. Gene ontology only detected enrichment for the downregulated genes of G. vaginalis and 47 GO terms were associated with molecular functions, cellular components and biological processes, mainly metabolism. Hence, this work showed the adaptation of 3 BVAB when growing in a triple-species biofilm, with several genes being differentially expressed in all the species growing in a polymicrobial biofilm.
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Project description:Coexistence of polymicrobial organisms acted as biofilm communities has become more and more prominent that these interactions lead to an increased resistance against host immune responses and antimicrobial agents, especially bacterial-fungal interactions. Klebsiella pneumoniae and Candida albicans are one of the interesting candidates acted as interkingdom biofilms. K. pneumoniae and C. albicans (KP+CA) presented the interkingdom biofilms with synergistic interaction through molecular analysis and confocal fluorescent imaging with z-stack thickness in vitro experiments. Both of an acute pneumonia model and in vitro neutrophil responses demonstrated that K. pneumoniae infection performed the highest severity when compared to mixed K. pneumoniae and C. albicans and alone C. albicans infections through various biomarkers and the responsiveness of neutrophils. Consequently, this study indicated that polymicrobial with bacterial-Candida infection may less severe than single-bacterial infection.
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Project description:Enterococcus faecalis is a commensal bacterium in the gastrointestinal tract (GIT) of humans and other organisms. Outside of the GIT, E. faecalis can cause infections in root canals, wounds, surgical sites, the urinary tract, and on heart valves. Many of these infections can be polymicrobial, and a variety of toxins and nutritional signals influence interactions between E. faecalis and other microbes. Like other bacteria, E. faecalis metabolizes arginine through the arginine deiminase (ADI) pathway, which converts arginine to ornithine and releases ATP, ammonia, and CO2. E. faecalis arginine metabolism also affects virulence of other pathogens, such as Clostridioides difficile and Escherichia coli, during co-culture. E. faecalis may encounter elevated levels of arginine in the GIT or the oral cavity, where arginine is used as an additive in dental therapeutics to disrupt plaque formation and biofilm growth by oral streptococci. However, little is known about how E. faecalis responds to growth in arginine. To address this gap, we used RNAseq and additional in vitro assays to measure changes in growth, gene expression, and biofilm formation in arginine relative to control conditions. Here, we demonstrate that arginine decreases E. faecalis biofilm production and causes widespread differential expression of genes related to metabolism, nutrient transport, quorum sensing, and polysaccharide synthesis. Growth in arginine also increases aggregation of E. faecalis cultures and promotes decreased susceptibility to the antibiotics ampicillin and ceftriaxone. Together, this shows that E. faecalis undergoes global transcriptional changes during growth in arginine and provides a platform for better understanding of how the presence of arginine in the niches colonized by E. faecalis affects the physiology of this organism in addition to the virulence of surrounding microbes.
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