Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Structural and molecular properties of inclusion bodies of mumps virus

ABSTRACT: Structural and molecular properties of inclusion bodies of mumps virus

PROVIDER: PRJNA1114030 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR29099231_1.fastq.gz	Fastqsanger.gz
	SRR29099231_2.fastq.gz	Fastqsanger.gz
	SRR29099232_1.fastq.gz	Fastqsanger.gz
	SRR29099232_2.fastq.gz	Fastqsanger.gz
	SRR29099233_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Structural and molecular properties of inclusion bodies of mumps virus

Project description:Viral RNA synthesis of mononegaviruses occurs in the cytoplasmic membrane-less organelles called as inclusion body (IB). Here, we reported that the IBs of mumps virus (MuV), which is the causative agent of mumps and belongs to the family Paramyxoviridae, had properties of liquid organelles formed by liquid-liquid phase separation. Super-resolution microscopic analysis of the MuV IBs demonstrated that nucleocapsid and phospho-(P) proteins formed the cage-like structure and that viral polymerase was in a reticular pattern and partially co-localized with viral mRNA. In addition, we characterized host RNAs localized in the MuV IBs by a spatial transcriptome analysis and found that G-quadruplex motif sequences containing RNAs (G4-RNAs) were concentrated. In vitro phase separation assay showed that the G4-RNAs interacted with the P protein and enhanced condensation in the P droplets. Taken together, our data show that MuV generates the IBs with a characteristic cage-like structure and host G4-RNAs play an important role in the formation of MuV IBs

2024-10-31 | GSE268004 | GEO

Structural and molecular properties of mumps virus inclusion bodies.

Project description:Viral RNA synthesis of mononegaviruses occurs in cytoplasmic membraneless organelles called inclusion bodies (IBs). Here, we report that IBs of mumps virus (MuV), which is the causative agent of mumps and belongs to the family Paramyxoviridae, displayed liquid organelle properties formed by liquid-liquid phase separation. Super-resolution microscopic analysis of MuV IBs demonstrated that nucleocapsid and phospho (P)-proteins formed a cage-like structure and that the viral polymerase adopted a reticular pattern and colocalized with viral RNAs. In addition, we characterized host RNAs localized in MuV IBs by a spatial transcriptome analysis, and found that RNAs containing G-quadruplex motif sequences (G4-RNAs) were concentrated. An in vitro phase separation assay showed that the G4-RNAs interacted with the P protein and enhanced condensation in P droplets. Together, our data show that MuV generates IBs with a characteristic cage-like structure and host G4-RNAs play an important role in forming MuV IBs.

| S-EPMC11623304 | biostudies-literature

Mumps orthorubulavirus

Project description:Mumps virus overview

| PRJNA73799 | ENA

Mumps orthorubulavirus

Project description:Mumps orthorubulavirus Genome sequencing

| PRJNA394142 | ENA

Mumps orthorubulavirus

Project description:Mumps Virus Sequencing - Infectious Clone Studies

| PRJNA883737 | ENA

Transposon mutagenesis screening of Sendai virus and mumps virus

Project description:We report the genetic plasticity of Sendai virus and mumps virus. We introduced insertional mutation in the virus genome and checked fitness by comparing distribution of mutans in passage 1 and passage 2.

2019-02-01 | GSE125257 | GEO

growth competition assay of Sendai virus and mumps virus

Project description:We evaluate the individual growth of 8 mutants of Sendai virus in the competitive condition under the cultured cells. We also evalate the growth of 6 mutants of mumps virus in the similar competitive condition.

2019-03-21 | GSE128625 | GEO

Mumps orthorubulavirus

Project description:Sequencing of Vaccine Preventable Disease Agents - Mumps

| PRJNA73019 | ENA

P-bodies dynamics of hESCs by unveiling P-bodies properties of hESC and hESC-derived mesodermal cells

Project description:P-bodies dynamics of hESCs by unveiling P-bodies properties of hESC and hESC-derived mesodermal cells

| PRJNA1033589 | ENA

Mumps orthorubulavirus isolate:20170608_BC01

Project description:Mumps rubulavirus Genomes from 2016 Arkansas Outbreak

| PRJNA517140 | ENA