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IgD expressed in nucleus of Pro-B cells promotes Pro-B cells proliferation by regulating E2F3 expression [ChIP-seq]

ABSTRACT: IgD expressed in nucleus of Pro-B cells promotes Pro-B cells proliferation by regulating E2F3 expression [ChIP-seq]

PROVIDER: PRJNA1114672 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29125840_1.fastq.gz	Fastqsanger.gz
	SRR29125840_2.fastq.gz	Fastqsanger.gz
	SRR29125841_1.fastq.gz	Fastqsanger.gz
	SRR29125841_2.fastq.gz	Fastqsanger.gz
	SRR29125842_1.fastq.gz	Fastqsanger.gz

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IgD expressed in nucleus of Pro-B cells promotes Pro-B cells proliferation by regulating E2F3 expression [ChIP-seq]

Project description:Immunoglobulin D (IgD) has historically been considered as a surface marker of mature B cell with its specific function being undefined. Until now, no evidence had been presented to suggest that IgD is also expressed in pro-B cells. Here, we developed a mouse model with a targeted deletion of IgD, and assessed the production of the IgM, IgG and IgA, as well as the generation of the antigen specific antibodies. The findings indicated no significant differences in these Ig levels between wild type and IgD-deficient mice. However, we observed a notable reduction in the number of mature B cells, which led us to the surprising discovery that this decrease in B cell count begins at the pro-B cell stage. More significantly, we identified that IgD, in its intact tetrapeptide structure, is expressed in the nucleus of pro-B cells. Functionally, IgD appears to promote the proliferation of pro-B cells. Mechanically, IgD exhibits a transcription factor-like activity, and directly binds to the promoter region of E2f3, a pro-proliferative transcription factor. IgD drives the expression of E2f3, thereby promoting the proliferation of pro-B cells. This novel insight into the physiological significance of IgD in B cell development has important implications for our understanding of immune system function.

2025-05-22 | GSE268102 | GEO

RNA-seq analysis of splenic follicular IgD low and IgD high, and marginal zone B cells

Project description:RNA sequencing was performed to determine the uniqueness of splenic follicular IgD low B cells compared to splenic follicular IgD high and marginal zone B cells.

2018-12-03 | GSE111911 | GEO

Microarray analysis of splenic naive IgD+CD27- and IgD+CD27+ B cell subsets cells from adult humans

Project description:RNA microarray profiling analysis was performed on splenic B cell subsets: “IgD+CD27-” (naive B cells) and “IgD+CD27+” (MZB B cells) isolated from splenic samples of 6 adults

2021-09-23 | E-MTAB-9620 | biostudies-arrayexpress

RNA sequencing of B cell subsets (CD11c hi IgD+ B cells, CD11c hi IgD- B cells, Memory B cells and Naïve B cells) from healthy subjects and subjects with Systemic lupus erythematosus (SLE) or Rheumatoid arthritis (RA)

Project description:CD11c+ B cells (IgD+ and IgD-) are pathogenic B cells expanded in autoimmune disease. The purpose of this study is to identify the pathways unique to IgD+ CD11c B cells and IgD- CD11c B cells.

2018-02-23 | GSE110999 | GEO

Role of IgD in B cell anergy

Project description:To define the global landscape of mRNA changes in anergic B cells, and how this affected by surface IgD, mature CD93-CD23+ HEL-binding B cells were sorted from spleens of independent transgenic mice of the following genotypes: MD4, naïve B cells coexpressing IgM and IgD; MD4:ML5, anergic B cells coexpressing IgM and IgD; MM4, naive B cells expressing only IgM; MM4:ML5, anergic B cells expressing only IgM; DD6, naive B cells expressing only IgD; DD6:ML5, anergic B cells expressing only IgD.

2016-09-30 | GSE82091 | GEO

RNA-seq analysis of splenic follicular IgD low and IgD high, and marginal zone B cells

Project description:RNA-seq analysis of splenic follicular IgD low and IgD high, and marginal zone B cells

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Extrafollicular IgD+ B cells Generate IgE Antibody Secreting Cells in the Nasal Mucosa

Project description:Here we describe the dynamics underlying the generation of IgE-antibody secreting cells (ASC) in human nasal polyps (NP), mucosal tissues rich in ASC without germinal centers (GC). Using VH next generation sequencing, we identified an extrafollicular (EF) mucosal IgD+ naïve-like intermediate B cell population with high connectivity to the mucosal IgE ASC. Mucosal IgD+ B cells, express germline epsilon transcripts and predominantly co-express IgM. However, a small but significant fraction co-express IgG or IgA instead which also show connectivity to ASC IgE. Phenotypically, NP IgD+ B cells display an activated profile and molecular evidence of BCR engagement. Transcriptionally, mucosal IgD+ B cells reveal an intermediate profile between naïve B cells and ASC. Single cell IgE ASC analysis demonstrates lower mutational frequencies relative to IgG, IgA, and IgD ASC consistent with IgE ASC derivation from mucosal IgD+ B cell with low mutational load. In conclusion, we describe a novel mechanism of GC-independent, extrafollicular IgE ASC formation at the nasal mucosa whereby activated IgD+ naïve B cells locally undergo direct and indirect (through IgG and IgA), IgE class-switch.

2021-05-13 | GSE174349 | GEO

Comparisons of Nasal Polyp ASC, Nasal polyp IgD+ (naïve-like) B cells, and Blood naïve B cells

Project description:Here we investigate the transcriptional landscapes of nasal polyp IgD+ (naïve-like) B cells, nasal polyp ASC, and blood naïve B cells using RNA-seq. These data found that nasal polypP IgD+ naïve-like B cells are activated and similar to nasal polyp ASC and distinct from circulating B cells in the blood.

2021-05-20 | GSE174681 | GEO

Murine IgD+B Cells transcriptome analysis -untreated and when stimulated with LPS

Project description:Transcriptome comparison of untreated murine IgD+B cell with LPS treated IgD+B cell B-lymphocytes constitute an important aspect of mammalian immune systems by virtue of their ability to produce highly specific antibodies in response to foreign antigens and pathogens. When B-lymphocytes encounter the particular antigen that they are responsive to, they differentiate into two types of cells: short-lived antibody-secreting plasma cells and long-lived memory cells. While the plasma B cells are responsible for the rapid resolution of a current infection or immune challenge, the memory B cells are responsible for mounting a rapid response to subsequent exposures. Previous work had demonstrated that treatment of mice with a single dose of anti-CD40 at the time of immunization lead to improved secondary responses, suggesting an enhancement of the memory B cell pool. We are currently studying the molecular mechanisms underlying the generation of memory B cells, using CD40 signalling as a tool. We have carried out a Microarray analyses of genome wide gene expression patterns in naÃ¯ve B-lymphocytes following CD40 signalling, over multiple time points. Analysis used untreated IgD+B cells as control for comparison to LPS treated cells as test. Indirect comparisons were made across multiple arrays with raw data pulled from different channels for data analysis and comparison to the control data.

2012-12-25 | E-GEOD-30546 | biostudies-arrayexpress

Extrafollicular IgD+ B cells Generate IgE Antibody Secreting Cells in the Nasal Mucosa

Project description:Extrafollicular IgD+ B cells Generate IgE Antibody Secreting Cells in the Nasal Mucosa

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