Project description:This study aims to delineate the effects of AXL deletion on kidney development and cellular heterogeneity by analyzing single-cell RNA transcriptomic datasets from 11-week-old male Axl wild type (WT) and heterozygous (het) knockout mice.
Project description:Comparison of kidneys of 4 different Pept2 KO individuals (GSE1585), pool of them and kidneys of WT individuals versus another WT individual (GSE1586). Keywords = Pept2 Keywords = Slc15a2 Keywords = kidney Keywords = KO Keywords = WT Keywords = pool Keywords = pooling Keywords = normal variatons Keywords = C57BL/6 Keywords = 129Sv Keywords: other
Project description:To identify transcriptomic differences in interscapular brown adipose tissue depots from HFD-challenged wild-type (WT) vs. Axl KO (whole-body Axl Receptor knockout) mice
Project description:Mincle (Macrophage inducible C-type lectin) is a pattern recognition receptor expressed in innate immune cells and can sense cell death, suggesting a role in sterile inflammation. We induced renal ischemia–reperfusion injury to KO and WT mice and collected the kidneys to compare the gene expression. Microarray analysis revealed that the overlap between the genes upregulated in injured kidney vs. sham kidney in WT mice and the genes downregulated in injured kidney in KO mice vs. WT mice included inflammation-related pathways on day 3.
Project description:AXL is activated by its ligand GAS6 and is expressed in triple-negative breast cancer cells. We report that AXL is also detected in HER2+ breast cancer specimens where its expression correlates with poor patients’ survival. Using murine models of HER2+ breast cancer, AXL, but not Gas6, was found essential for metastasis. We determined that AXL is required for intravasation, extravasation and growth at the metastatic site. AXL is expressed in HER2+ cancers displaying EMT signatures and contributes to sustain EMT in murine tumors. Interfering with AXL in patient-derived xenograft impaired TGF-β-induced cell invasion. Lastly, pharmacological inhibition of AXL decreased the metastatic burden of mice developing HER2+ breast cancer. Our data identify AXL as a potential co-therapeutic target during the treatment of HER2+ breast cancers to limit metastasis.
Project description:Estrogen-related receptor (ERR) alpha is an orphan nuclear receptor highly expressed in the kidneys. ERRalpha is implicated in renal sodium and potassium homeostasis and blood pressure regulation. We used microarray analysis to identify differentially expressed genes in ERR alpha knockout mice kidneys versus wild-type. The results provide insight on the roles of ERRalpha in the kidney. Three biological replicates of WT and ERRaKO were performed, for a total of 6 samples. 2-3 month old males of each genotype were used.
Project description:Transcriptional profiling of whole kidneys from Six2CreEGFP mice without (WT) or with (KO) homozygously floxed DOT1 alleles at the age of embryonic day 16.5. This experiment aimed to uncover the genome-wide alternation in gene expression resulting from the removal of DOT1 gene in the nephron progenitor population (Six2 positive) and successive changes to the series of events in kidney development.
Project description:Either WT or muMT KO mice were infected with UPEC trans urethrealy twice 1 hour apart and culled 6 hours later. Kidneys were subsequently perfused, RNA extracted and analysed via RNASeq