Project description:wt plants (ws) and opr3 mutant plants were wounded Half of the rosette leaves of 6 weeks old plants were wounded by clamping a tweezers across the midvein. RNA was extracted from control and systemic leaves 2 h after wounding, and was subject to affymetrix ATH1 chip. one repeat: wt control, wt systemic wounding, opr3 control, opr3 systemic wounding
Project description:Transcriptional profiling of Arabidopsis thaliana wild type (WT) comparing MD (mechanical damage) and HW (herbivore wounding). The differences in the biochemical responses to herbivory seen prompted us to search for less obvious differences between treatments using gene expression profiling. Biological replicates: 4
Project description:This study aims to identify genes which help to understand similar underlying mechanism in the response to shade and wounding in Arabidopsis thaliana plants.
Project description:Transcriptional profiling of Arabidopsis thaliana wild type (WT) and a line mutated in genes encoding plasmodesmata (PD) proteins (PDKO3) comparing MD (mechanical damage) and HW (herbivore wounding). The differences in the biochemical responses to herbivory seen in the PDKO3 when compared to the WT line, together with the absence of growth/developmental phenotypes in the former, prompted us to search for less obvious differences between the lines using gene expression profiling.
Project description:Comparison of gene expression between shoots of root-wounded seedlings and shoots of control seedlings in Arabidopsis. We identified wounding-induced early (30 min) and late (360 min) root to shoot responsive genes (RtS).