Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Rhipicephalus microplus strain:BME/CTVM6

ABSTRACT: SFTSV infected BME/CTVM6 cell lines from Rhipicephalus microplus tick.

PROVIDER: PRJNA1116706 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR29948300_1.fastq.gz	Fastqsanger.gz
	SRR29948300_2.fastq.gz	Fastqsanger.gz
	SRR29948301_1.fastq.gz	Fastqsanger.gz
	SRR29948301_2.fastq.gz	Fastqsanger.gz
	SRR29948302_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Transcriptomic analysis of Rhipicephalus microplus hemocytes from female ticks infected with Babesia bovis or Babesia bigemina

Project description:To understand the effect of Babesia infection on Rhipicephalus microplus hemocyte gene expression, we performed high throughput RNA-sequencing using samples collected from Rhipicephalus microplus uninfected tick hemolymph and infected with either Babesia bigemina or Babesia bovis. We evaluated gene expression differences that may be attributed to tick immune defense to babesial infection.

2023-12-31 | GSE243493 | GEO

Comparative microarray analysis of midgut tissues from feeding Rhipicephalus microplus and Rhipicephalus decoloratus adult females

2015-01-31 | GSE55826 | GEO

Transcriptional and proteomic dynamics of severe fever with thrombocytopenia syndrome virus infection in Rhipicephalus microplus cells identify antiviral host factors.

Project description:Ticks are vectors of arboviruses in many parts of the world. The rising incidence and emergence of tick-borne arboviral infections across human populations indicates that further transmission control strategies including those based on vectors, will be required to reduce the burden of disease. However, arbovirus-tick interactions at the cellular level remain poorly understood in general, and particularly neglected for negative strand RNA arboviruses. In this study we developed a proteomics informed by transcriptomics approach to characterize the cellular response of Rhipicephalus microplus-derived cell cultures to infection with the tick-borne pathogen severe fever with thrombocytopenia syndrome virus (SFTSV, Phenuiviridae). For this, we generated the first de novo transcriptomes and confirmed proteomes of SFTSV- or mock-infected tick cell cultures derived from a vector species that transmits the virus in nature. Through comprehensive annotation of genes, proteins and pathway analysis, we identified core host responses and regulatory processes mediated in response to SFTSV infection. Moreover, examining the interactome of the virally encoded nucleoprotein (N) allowed us to integrate host responses with the analysis of cellular factors required for viral replication. The influence of specific host genes on SFTSV replication was systematically assessed through dsRNA-mediated gene silencing. This functional genomics approach pinpointed two tick-derived RNA helicases as critical antiviral factors capable of restricting SFTSV infection: the DexD/box helicase (DHX9) and the Up-Frameshift Protein 1 (UPF1). Collectively, our findings enrich the repository of resources available for understanding the antiviral response to SFTSV infection in Rh. microplus vector cells and support the identification of SFTSV-antiviral restrictions factors.

2025-05-26 | PXD052311 | Pride

Comparative microarray analysis of midgut tissues from feeding Rhipicephalus microplus and Rhipicephalus decoloratus adult females

Project description:Transcriptional profiling of midgut tissues isolated from Rhipicephalus microplus and Rhipicephalus decoloratus females at day 20 post infestation. This enabled the identification of transcripts that are species-specific or shared between the two tick species tested. A direct comparison (Balanced block) was performed: Midgut tissues collected on day 20 (post infestation) from R. microplus was compared to that of R. decoloratus. Biological replicates: 3 ; Technical replicates: 1.

2015-01-31 | E-GEOD-55826 | biostudies-arrayexpress

Transcriptional responses in bovine skin during blood feeding of the cattle tick, Rhipicephalus microplus.

Project description:We examined gene expression induced by Rhipicephalus microplus bites on host skin of tick-resistant and tick-susceptible breeds of bovines, Nelore and Holstein respectively, when they underwent a primary infestation.

2017-04-02 | E-MTAB-4908 | biostudies-arrayexpress

Peripheral WBC from Brahman and Holstein-Friesian cattle infested with the cattle tick Rhipicephalus microplus

Project description:This trial was undertaken to examine the perhipheral cellular and antibody response of cattle following infestation with the cattle tick, Rhipicephalus microplus. The information from the Affymetrix gene expression data is used to complement other measurements of immune function such as cellular subset composition and antibody response in cattle of high (Brahman) and low (Holstein-Friesian) resistance to the cattle tick. Keywords: Disease state analysis

2008-11-26 | GSE13725 | GEO

SAGE in Rhipicephalus (Boophilus) microplus following OP treatment of larvae from OP resistant/susceptible strains.

Project description:Organophosphate resistant and susceptible tick larvae from laboratory strains of the southern cattle tick, Rhipicephalus (Boophilus) microplus were exposed to low doses of the organophosphate (OP) acaricide, coumaphos. Serial Analysis of Gene Expression (SAGE) was used to analyze differential gene expression in response to OP treatment and to compare the responses in OP-treated and untreated resistant and susceptible tick larvae. ** note: Contact person is Felix D. Guerrero. email: felix.guerrero@ars.usda.gov Keywords: SAGE, acaricide response, organophosphate.

2008-05-14 | GSE10109 | GEO

Microarray analysis of gene expression in acaricide exposed Rhipicephalus (Boophilus) microplus.

Project description:A R. microplus microarray was used to study differential gene expression in acaricide exposed larvae from an amitraz-resistant strain. The acaricide treatments were: organophosphate (OP), pyrethroid, ivermectin, and amitraz. The microarrays contained over 13,000 probes corresponding to each member of R. microplus gene index ESTs previously described (http://compbio.dfci.harvard.edu/tgi/cgi-bin/tgi/gimain.pl?gudb=b_microplus). Serial analysis of gene expression (SAGE) data from the OP treated R. microplus was used to verify the OP microarray data. The expression profiles of selected transcripts were verified by real time PCR. Among the significantly differentially expressed genes, were a tick legumain, involved in blood digestion, gluthathione S-transferase (GST), a detoxification enzyme involved in pesticide resistance, acyltransferase, several putative salivary sulfotransferases, and a glutamate receptor. **Note: contact person: Felix D. Guerrero email: felix.guerrero@ars.usda.gov Keywords: Rhipicephalus (Boophilus) microplus, acaricide resistance genes, organophosphates OP, microarrays, detoxification enzymes.

2008-12-31 | GSE10171 | GEO

Rhipicephalus microplus

Project description:Rhipicephalus microplus overview

| PRJNA46693 | ENA

SAGE in Rhipicephalus (Boophilus) microplus following OP treatment of larvae from OP resistant/susceptible strains.

2008-05-14 | E-GEOD-10109 | biostudies-arrayexpress