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RNA sequencing (RNA-seq) of Streptococcus pyogenes - group A Streptococcus (GAS) S119 strain in the presence or absence of asparagine (Asn) grown in a chemically defined medium (CDM) (van de Rijn, I. & Kessler, R.E. (1980).

ABSTRACT: RNA sequencing (RNA-seq) of Streptococcus pyogenes - group A Streptococcus (GAS) S119 strain in the presence or absence of asparagine (Asn) grown in a chemically defined medium (CDM) (van de Rijn, I. & Kessler, R.E. (1980).

PROVIDER: PRJNA1117490 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29208030.fastq.gz	Fastqsanger.gz
	SRR29208031.fastq.gz	Fastqsanger.gz
	SRR29208032.fastq.gz	Fastqsanger.gz
	SRR29208033.fastq.gz	Fastqsanger.gz
	SRR29208034.fastq.gz	Fastqsanger.gz

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RNA sequencing (RNA-seq) of Streptococcus pyogenes - group A Streptococcus (GAS) S119 strain in the presence or absence of asparagine (Asn) grown in a chemically defined medium (CDM) (van de Rijn, I. & Kessler, R.E. (1980).

Project description:The study was conducted to test the global effect of Asn on the transcriptome of the GAS S119 strain. Transcriptome levels in the presence or absence of Asn of cultures harvested at OD600 = 0.7 were compared. We report here that a global differential expression of genes belonging to diverse functional categories, including virulence, metabolism, transporters, regulatory proteins, kinases, hypothetical proteins, and other categories.

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RNA-seq to investigate differential expression of serotype emm1 Streptococcus pyogenes strains S119 and S126

Project description:The human pathogen Streptococcus pyogenes, or group A streptococcus, is responsible for mild infections to life-threatening diseases. We previously have performed the transcription start site profiling of a Streptococcus pyogenes emm1 strain, strain S119, an invasive strain isolated from a blood culture. Here, we perform strand-specific RNA-seq experiments to complete this characterization and analyze the global coverage and the differential expression in growth medium complemented or not with 15 mM MgCl2. In addition we compare these results to those obtained with a related strain, strain S126, corresponding to a colonization sample, that differs from S119 by only one mutation in the two-component regulator of virulence CovRS.

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Project description:Comparison of Streptococcus pneumoniae D39 wild-type grown in CDM+10 mM arginine compared to D39 wild type grown in CDM + 0.05 mM arginine to define the genome-wide transcriptional response to arginine. Details described in Kloosterman TG and Kuipers OP. ArgR1 and AhrC Mediate Arginine-Dependent Regulation of Arginine Acquisition- and Virulence Genes in the Human Pathogen Streptococcus pneumoniae. JBC 2011

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Streptococcus pyogenes strain S119

Project description:Genome sequence of Streptococcus pyogenes strain S119

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Project description:Comparison of the Streptococcus pneumoniae D39 wild-type in CDM Plus 0mM CO and CDM plus 0,5 mM Co

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Streptococcus pneumoniae D39 wild-type grown in CDM+10 mM arginine compared to D39 wild type grown in CDM + 0.05 mM arginine

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argR1 mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + 10 mM arginine

Project description:Comparison of Streptococcus pneumoniae D39 argR1 mutant compared to D39 wild type in CDM with 10 mM arginine to define the regulon of the ArgR1 regulator under this condition. Details described in Kloosterman TG and Kuipers OP. ArgR1 and AhrC Mediate Arginine-Dependent Regulation of Arginine Acquisition- and Virulence Genes in the Human Pathogen Streptococcus pneumoniae. JBC 2011

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ahrC mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + 10 mM arginine

Project description:Comparison of Streptococcus pneumoniae D39 ahrC mutant compared to D39 wild type in CDM with 10 mM arginine to define the regulon of the AhrC regulator under this condition. Details described in Kloosterman TG and Kuipers OP. ArgR1 and AhrC Mediate Arginine-Dependent Regulation of Arginine Acquisition- and Virulence Genes in the Human Pathogen Streptococcus pneumoniae. JBC 2011

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argR1-ahrC mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + 10 mM arginine

Project description:Comparison of Streptococcus pneumoniae D39 argR1-ahrC mutant compared to D39 wild type in CDM with 10 mM arginine to define the regulons of the ArgR1 and AhrC regulators under this condition. Details described in Kloosterman TG and Kuipers OP. ArgR1 and AhrC Mediate Arginine-Dependent Regulation of Arginine Acquisition- and Virulence Genes in the Human Pathogen Streptococcus pneumoniae. JBC 2011

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