Project description:To find out differently expressed circRNAs during Orf virus infection of GSF cells and their potential roles in response to ORFV infection
Project description:Contagious ecthyma (Orf) is a contagious disease with worldwide distribution, caused by the epitheliotropic Orf virus (ORFV), a member of the genus Parapoxvirus. In the current study, we collected the oral mucosa tissues samples (T0, T3, T7 and T15) from sheep at 0, 3, 7 and 15 days post ORFV infection, respectively. To explore the changes of comprehensive transcriptome of host cells from oral mucosa tissues post ORFV infection, the RNA-seq transcriptome comparisons were performed on these samples. It showed that 1928, 3219 and 2646 differentially expressed genes (DEGs) were identified among T3 vs. T0, T7 vs. T0, T15 vs. T0 respectively. Through Gene Ontology (GO) analyses of the DEGs from these comparisons, it revealed that ORFV provoked the vigorous immune response of the host cells during the early stage of infection. Moreover, GO and network analysis revealed that positive and negative regulative mechanisms of apoptosis worked as whole in the host cells, in order to reach a homeostasis of oral mucosa tissues.
Project description:This project focuses on the proteomic analysis of biofilm formation in Aeromonas hydrophila LP-2. By comparing the wild-type strain and the orf-2341 deletion mutant, we aim to identify biofilm-associated proteins and explore the role of orf-2341 in outer membrane protein assembly and compensatory pathways. The study utilizes advanced proteomics techniques, including LC-MS/MS, to uncover differential protein expression patterns.
