Project description:Single-cell RNA-sequencing analyses were performed on pancreatic tumors from KPPC mice and PPSSC mice, so as to identify the changes of tumor immune microenvironment upon Trp53 and Smad4 deletion without KRAS mutation.
Project description:RNA-sequencing analyses were performed on cancer cell lines from KPPC and PPSSC pancreatic tumors, so as to identify the changes of tumor immune microenvironment upon Trp53 and Smad4 deletion without KRAS mutation.
Project description:We pooled three 8 week-old KPPC WT (n=3), MAP3K7ff KPPC (n=3) and MAP3K7fwt KPPC (n=3) mice pancreatic tumor respectively into one sample, prepared single cell suspension and performed 10X genomics scRNASeq to assess transcriptomic changes all cell populations
Project description:We pooled three KPPC mice tumors into one sample for vehicle (n=3) or Takinib (n=3), prepared single cell suspension and performed 10X genomics scRNASeq to assess transcriptomic changes all cell populations
Project description:RNA sequencing analysis on two primary pancreatic cancer cell lines from transgenic mice: (1) cancer-collagen1 knockout cell line from KPPC;Col1pdxKO cancer-collagen1 knockout tumor and (2) control pancreatic cancer cell line from KPPC tumor
Project description:Here, we sought to evaluate the impact of KrasG12D and KrasG12C inhibitors on pancreatic cancer cells. A murine pancreatic cancer cell line with KrasG12D mutation was treated with MRTX1133 (KrasG12D inhibitor) and MRTX849 (KrasG12C inhibitor) and scRNA-seq performed to evaluate transcriptional changes. We further evaluated transcriptional changes in cancer cells, as well as in the tumor microenvironment of immunodeficient (NSG) and immunocompetent (C57BL6/J) mice orthotopically implanted with KPC689 cells and spontaneous KPPC tumors in response to MRTX1133 and MRTX849.
Project description:We treated KPPC cells with DMSO and EphA2 inhibitor for 24 hours and performed bulk RNAseq to assess transcriptomic changes KPPC cells
Project description:Single-cell RNA-sequencing analyses were performed on unfractionated live cell mixtures from pancreatic tumors of KPC mice and wild-type mice to detect different myCAF subpopulations