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ABSTRACT: Points of departure for an algal species (Raphidocelis subcapitata) exposed to 22 per- and polyfluoroalkyl substances

PROVIDER: PRJNA1119425 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29263100.fastq.gz	Fastqsanger.gz
	SRR29263200.fastq.gz	Fastqsanger.gz
	SRR29263300.fastq.gz	Fastqsanger.gz
	SRR29263400.fastq.gz	Fastqsanger.gz
	SRR29263500.fastq.gz	Fastqsanger.gz

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Points of departure for an algal species (Raphidocelis subcapitata) exposed to 22 per- and polyfluoroalkyl substances

Project description:Due to their environmental prevalence, persistence, and potential toxicity, per- and polyfluoroalkyl substances (PFAS) are contaminants of concern. However, except for a few well studied PFAS, hazard data required to evaluate their risk to aquatic ecosystems are limited. The present study used a 24-hour, high throughput assay to screen 22 PFAS with varying chain lengths and functional groups for effects on the transcriptome of a green algal species, Raphidocelis subcapitata. The tested PFAS included perfluoroalkyl carboxylic acids, perfluoroalkyl carboxylic acid ethers, alcohols, sulfonic acids, and sulfonamides. Algae were exposed in a 96-well microplate format to 8 concentrations (nominally, 100 to 0.03 uM) of each PFAS, in a 1/2 log dilution series. Analytical measurements of PFAS concentration were made at the initiation of exposure and after 24 hours. In addition to traditional endpoints (i.e., biomass, viability, and chlorophyll), RNA was extracted for sequencing of the transcriptome. Concentration response curves were fit to the resulting transcriptomic data to calculate gene-specific benchmark concentrations. The distribution of benchmark concentrations was subsequently used to calculate a transcriptomic point of departure (tPOD) which represents a concentration below which no concerted molecular change is detected in response to PFAS exposure. The resulting tPODs were compared with traditional endpoints and environmentally detected concentrations of specific PFAS.

2025-06-06 | GSE269539 | GEO

Project description:Transcriptomics-based points of departure for Daphnia magna exposed to 18 per- and polyfluoroalkyl substances

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Raphidocelis subcapitata

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Project description:Derivation of transcriptomics-based points of departure for twenty per- or polyfluoroalkyl substances (PFAS) using a larval Pimephales promelas reduced transcriptome assay.

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Sertraline Hydrochloride Exposure Leads to Reactive Oxygen Species Burst in Model Microalgae Species (Raphidocelis subcapitata)

Project description:Microalgae play important ecological roles and serve as useful models for assessing impacts of environmental contaminants on lower trophic aquatic organisms. Sertraline, a commonly prescribed selective serotonin reuptake inhibitor (SSRI) found in urban waters, has been observed to induce chlorosis in Raphidocelis subcapitata close to environmentally relevant concentrations. This study estimated potentially hazardous concentrations of sertraline hydrochloride by deriving transcriptomic and metabolomic points of departure (tPODs and mPODs) below which chronic adverse effects would not be expected. Additionally, an Adverse Outcome Pathway describing the mechanism by which sertraline hydrochloride impacted R. subcapitata was proposed using a multi-omics approach. The mPOD derived from nontargeted metabolomics was comparable to the tPOD (455 µg/L vs 690 µg/L) and annotated dysregulated metabolites along with reduced photosynthetic capability indicated that uncoupling of the cyclic electron flow required for photosynthesis led to a reactive oxygen species (ROS) burst. This probable ROS burst altered cell membrane composition and downregulated genes associated with protoporphyrinogen IX and heme biosynthesis pathways. This research highlights the molecular mechanisms underlying pharmaceutical-induced chlorosis in a model microalgae species and demonstrates the utility of integrating metabolomics and transcriptomics for assessing the potential ecological risks of SSRIs.

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Transcriptomic analysis and cellular responses to nanoscale zero-valent iron in green microalga Raphidocelis subcapitata

Project description:Nanoscale zero valent iron (nZVI) is used to remediate aquifers polluted by organochlorines or heavy metals and was also suggested to eliminate harmful algal blooms. nZVI can therefore affect microorganisms in the vicinity of the application area, including microalgae. However, studies on early transcriptomic effects of microalgae after exposure to nZVI are rare. Here, we described the early physiological and transcriptomic response of the freshwater ecological indicator green microalga, Raphidocelis subcapitata ATCC 22662, to 100 mg/L of reactive nZVI and non-reactive nano-magnetite (nFe3O4). The combined effect of shading and the release of total iron from nZVI posed a short-term inhibition effect leading to 15 % of deformed cells and cytosol leakage, while cells viability increased after 24 h. nZVI triggered a more pronounced transcriptomic response with (7380 differentially expressed genes [DEGs]) compared to nFe3O4 (4601 DEGs) after 1 h. nZVI, but not nFe3O4 increased the expression of genes function in DNA repair and replication, while deactivated carbohydrate-energy metabolisms, mitochondria signaling, and transmembrane ion transport. This study highlights an early fate assessment of algal cells under nZVI and nFe3O4 exposure using next-generation risk assessment methods and will serve as valuable information for safe and sustainable application of nZVI in water remediation.

2023-06-06 | GSE234088 | GEO

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