Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA112017 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptional responses to nanoparticulate matter in human skin-derived cancer cells
| PRJNA134425 | ENA
Project description:Transcriptional responses of human colon and liver cancer cells to TCF inhibition.
| PRJNA200691 | ENA
Project description:This study evaluated transcriptional effects of particles smaller than 100 nm of TiO2 and ZnO. Based on previous data in colon cancer cells (GSE14910), we evaluated HaCaT and Sk Mel-28 cells for transcriptional responses to 1 and 5 ug/cm2 ZnO, or 5 and 10 ug/cm2 TiO2. No particle controls were also included. Again, the most pronounced transcriptional response resulted from ZnO treatement with little responses to TiO2. We identified increased protein stress responses, decreased regulation of transcription, and responses to Zn ions. We did not observe the protein stress response and regulation of transcription with soluble Zn. Keywords: skin-derived cancer cells - response to ZnO nanoparticulate Two skin-derived cancer cell types (HaCaT and SK Mel-28) were treated with media containing the nanoparticulate, ZnCl2, or ZnO separated from the HaCaTs by a Transwell insert, and RNA was collected after 4 hrs. Generally, the RNA from 4 independent samples were combined for one microarray.
2011-11-01 | E-GEOD-25167 | biostudies-arrayexpress
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.
2011-02-19 | GSE20463 | GEO
Project description:This study evaluated transcriptional effects of particles smaller than 100 nm of carbon black, SiO2, Al2O3, TiO2, ZnO and Fe203. Since there is concern that inflammation may increase the uptake and/or toxicity of ultrafine particles, we evaluated the transcriptional responses without or with a TNFα pretreatment to mimic an inflammatory state. The most pronounced transcriptional response resulted from ZnO treatement, another response was to TNFα pre-treatment. We identified stress responses, responses to Zn ions, but did not observe a consistent proinflammatory response as evaluated by Gene Ontology of the genes that altered expression most as identified by significance analysis. Keywords: colon cancer cells - response to distinct nanoparticulate Two colon cancer cell types (RKO and CaCo-2) were pretreated with 100 ng/ml BSA or TNFα for 1 hr, the media was exchanged and with media containing the nanoparticulate, and RNA was collected after 4 hrs. Generally, the RNA from 4 independent samples were combined for one sample on the microarray, the exceptions were the TNFα treated controls where 6 independent samples were combined in 2 sets of 3 for the microarray labeling and hybridizations.
2010-12-01 | E-GEOD-14910 | biostudies-arrayexpress
Project description:This study evaluated transcriptional effects of particles smaller than 100 nm of TiO2 and ZnO. Based on previous data in colon cancer cells (GSE14910), we evaluated HaCaT and Sk Mel-28 cells for transcriptional responses to 1 and 5 ug/cm2 ZnO, or 5 and 10 ug/cm2 TiO2. No particle controls were also included. Again, the most pronounced transcriptional response resulted from ZnO treatement with little responses to TiO2. We identified increased protein stress responses, decreased regulation of transcription, and responses to Zn ions. We did not observe the protein stress response and regulation of transcription with soluble Zn. Keywords: skin-derived cancer cells - response to ZnO nanoparticulate
2011-11-01 | GSE25167 | GEO
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression. Two-condition experiment, Normoxic MSCs vs. Hypoxic MSCs.
2011-02-19 | E-GEOD-20463 | biostudies-arrayexpress
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
2010-09-25 | GSE24338 | GEO
Project description:This study evaluated transcriptional effects of particles smaller than 100 nm of carbon black, SiO2, Al2O3, TiO2, ZnO and Fe203. Since there is concern that inflammation may increase the uptake and/or toxicity of ultrafine particles, we evaluated the transcriptional responses without or with a TNFα pretreatment to mimic an inflammatory state. The most pronounced transcriptional response resulted from ZnO treatement, another response was to TNFα pre-treatment. We identified stress responses, responses to Zn ions, but did not observe a consistent proinflammatory response as evaluated by Gene Ontology of the genes that altered expression most as identified by significance analysis. Keywords: colon cancer cells - response to distinct nanoparticulate
2010-12-01 | GSE14910 | GEO
Project description:Gene methylation profiling of immortalized human mesenchymal stem cells comparing HPV E6/E7-transfected MSCs cells with human telomerase reverse transcriptase (hTERT)- and HPV E6/E7-transfected MSCs. hTERT may increase gene methylation in MSCs. Goal was to determine the effects of different transfected genes on global gene methylation in MSCs.
2011-08-25 | GSE23936 | GEO