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ABSTRACT: RNAseq of Diuraphis noxia and Triticum aestivum to investigate D. noxia virulence

PROVIDER: PRJNA1120216 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29288432_1.fastq.gz	Fastqsanger.gz
	SRR29288432_2.fastq.gz	Fastqsanger.gz
	SRR29288433_1.fastq.gz	Fastqsanger.gz
	SRR29288433_2.fastq.gz	Fastqsanger.gz
	SRR29288434_1.fastq.gz	Fastqsanger.gz

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Diuraphis noxia

Project description:Diuraphis noxia overview

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Whole-body transcriptome mining for candidate effectors from Diuraphis noxia

Project description:RNA was extracted from a pooled sample of salivary glands that were excised from adult Diuraphis noxia aphids of two biotypes SA1 and SAM2.0. South African D. noxia biotype SA1 is known to be the least virulent aphid, while its offspring, the South African D. noxia biotype SAM2.0 is a newly developed biotype that is capable of feeding on several resistant cultivars. The overall purpose of the experiment was to establish a baseline availability of transcripts contained in the salivary glands of D. noxia aphids as well as help improve on current genome assemblies. In total, 200 glands were excised from both biotypes feeding on various wheat hosts (Tugela, Tugela Dn1, Gamtoss R, SST387). Aphid glands were excised in PBS buffer and stored in RNA-later until RNA extraction with a Qiagen Rneasy kit could commence. Library preparation for sequencing was performed using an Illumina TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Sample Preparation Guide, Part # 15031047 Rev. E protocol. The single library was then sequenced on a NovaSeq6000 system. De novoassembly was performed making use of the Trinity package.

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Wheat Freeze-Tolerance

Project description:Comparison of gene expression levels in non-hardened (NH), cold-hardened (CH) and subzero-hardened (SZH) Triticum aestivum L. cv Jackson crowns with Affymetrix GeneChip Barley Genome Array. Keywords = wheat Keywords = crowns Keywords = cold tolerance Keywords = freezing resistance Keywords = Triticum aestivum L. cv Jackson Keywords: repeat sample

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Diuraphis noxia

Project description:Diuraphis noxia Transcriptome or Gene expression

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Project description:Diuraphis noxia Genome sequencing and assembly

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Transcriptional analyses of wheat responses to the necrotrophic effector SnTox3

Project description:Wheat (Triticum aestivum) was infiltrated with the Stagonospora nodorum effector protein SnTox3 to identify differentially regulated genes.

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Project description:HiFi Long read sequencing of Diuraphis noxia

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RNAseq of roots and leaves of tender wheat (Triticum aestivum cv chinese Spring) during interactions with mycorhizal fungi (Funneliformis mossae) with and without a pathogen attack by Xanthomonas translucens

Project description:We monitored by RNAseq the transcriptomic response of roots and leaves of Triticum aestivum cv chinese Spring during a long term interaction with Funneliformis mossae (2 months) with or without a pathogen infection by infiltration of Xanthomonas translucens CFBP 2054. The control condition of roots and leaves wheat without mycorhizal fungi is in E-MTAB-5891 (material produced simultaneously and treated at the same time).

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Bacteria Associated with Russian Wheat Aphid (Diuraphis noxia) Enhance Aphid Virulence to Wheat

Project description:Phenotypic responses to biotic stresses are often studied as the interactions between two species; however, in the phytobiome, these responses frequently result from complex interactions involving several organisms. Here, we show that variation in chlorosis caused by Russian wheat aphid (Diuraphis noxia) feeding is determined, in part, by aphid-associated bacteria. Proteomic analysis of fluids injected into a sterile medium by the aphid during feeding indicate that 99% of the proteins are of bacterial origin. Of these, the greatest proportion are produced by bacteria in the order Enterobacteriales. Bacteria from five genera in four families that have the capacity to produce these proteins were isolated directly from aphids as well as from wheat leaves only after D. noxia feeding. By themselves or in combination, these bacteria were not virulent to wheat, even at high inoculum levels. Metagenomic analysis showed that the same five D. noxia-associated genera dominated the non-Buchnera component of the aphid microbiome, and that representation of these genera was reduced in aphids from colonies established after isolation of newborn nymphs from their mothers prior to feeding (‘isolated’ aphids). Isolation or treatment with antibiotics reduced bacterial numbers, and these aphids caused less feeding damage on wheat than non-isolated or non-antibiotic treated aphids. Our data show that bacterial proteins are a significant component of Russian wheat aphid saliva, that the bacteria producing these proteins are associated with aphids and plants fed upon by aphids, and that these aphid-associated bacteria facilitate aphid virulence to wheat.

2020-11-09 | PXD010663 | Pride

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