Project description:Dendrobium species are widely used in traditional Chinese medicine owing to their medicinal and nutritional value, yet the phytochemical diversity and molecular mechanisms underlying theirbioactivities remain poorly understood. To elucidate these traits, we performed an integrative multi-omics study combining metabolomics and transcriptomics on three species, D. huoshanense, D. officinale, and D. moniliforme from the Dabie Mountains in Central China, with emphasis on flavonoid metabolism, particularly apigenin derivatives. Among 470 shared metabolites, quantitative profiling revealed distinct accumulation patterns between stems and leaves. Transcriptomic analysis uncovered species- and tissue-specific regulatory networks governing flavonoid biosynthesis. Complementary HPLC-DAD fingerprinting of stems, leaves, roots, and flowers was established and rigorously validated, with 18 common peaks annotated. VIP analysis identified tissue-specific chemical markers for quality assessment. Apigenin-6-C-α-L-arabinoside-8-C-β-D-xyloside and apigenin-6-C-α-L-rhamnosyl-(12)-β-D- glucoside-8-C-α-L-arabinoside were predominant in D. huoshanense stems, while flowers accumulated high levels of flavonoids such as rutin and vicenin-1 and displayed superior anti-inflammatory activity. Comprehensive nutritional profiling quantified total carbohydrates, polysaccharides, monosaccharides, fatty acids, amino acids, minerals, and vitamins across plant organs. Machine learning integration of multi-omics data delineated key molecular signatures distinguishing the three Dendrobium species from the Dabie Mountains. Together, this work provides a multidimensional resource that advances the biochemical understanding of Dendrobium, supports species and tissue discrimination, and informs quality control and utilization of both medicinal and edible plant parts.

2026-06-28 | MTBLS14876 | MetaboLights

Maize gene expression during infection with Fusarium moniliforme

2009-12-16 | GSE19501 | GEO

iTRAQ proteomic analysis of stems and leaves from two alfalfa genotypes

Project description:To explore the molecular regulatory mechanisms of early stem and leaf development, proteomic analysis was performed on leaves and stems of F genotype alfalfa, with thin stems and small leaves, and M genotype alfalfa, with thick stems and large leaves.

2020-11-13 | PXD022535 |

Transcription profiling of maize gene expression during infection with Fusarium moniliforme

Project description:The fungal pathogen Fusarium moniliforme causes ear rot in maize. Ear rot in maize is a destructive disease globally caused by Fusarium moniliforme , due to decrease of grain yield and increase of risks in raising livestock by mycotoxins production. Plants have developed various defense pathways to cope with pathogens. We used microarrays to detail the global programme of gene expression during the infection process of Fusarium moniliforme in its host plant to get insights into the defense programs and the host processes potentially involved in plant defense against this pathogen. Experiment Overall Design: In two compared independent experiments plants were infected with the Fusarium moniliforme. Samples from infected bracts of resistant maize (Bt-1) as well as susceptible maize (Ye478) were taken at 4 days post infection. Samples from uninfected control plants were taken at the same time points. For example: R0 (control) and RT (treat) in Bt-1 and S0 (control) and ST (treat) in Ye478.

2010-01-19 | E-GEOD-19501 | biostudies-arrayexpress

Dendrobium chrysotoxum

Project description:RNA-seq raw reads of Dendrobium chrysotoxum flowers

| PRJNA864082 | ENA

Glycine max isolate:Roots,stems,leaves,flowers,pods and seeds | cultivar:Zhonghuang 13

Project description:Glycine max isolate:Roots,stems,leaves,flowers,pods and seeds | cultivar:Zhonghuang 13 Raw sequence reads

| PRJNA869516 | ENA