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USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication

ABSTRACT: USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication

PROVIDER: PRJNA1122641 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29362466.fastq.gz	Fastqsanger.gz
	SRR29362467.fastq.gz	Fastqsanger.gz
	SRR29362468.fastq.gz	Fastqsanger.gz
	SRR29362469.fastq.gz	Fastqsanger.gz

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Similar Datasets

USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication

Project description:Mammalian DNA replication relies on various DNA helicases and nuclease activities to ensure accurate genetic duplication, but how different helicase and nuclease activities are properly directed remains unclear. Here, we identify the ubiquitin-specific protease, USP50, as a chromatin-associated protein required to promote ongoing replication, fork restart, telomere maintenance, cellular survival following hydroxyurea or pyridostatin treatment, and suppression of DNA breaks near GC-rich sequences. We find that USP50 supports proper WRN: FEN1 localisation at or near stalled replication forks. Nascent DNA in cells lacking USP50 shows increased association of the DNA2 nuclease and RECQL4 and RECQL5 helicases and replication defects in cells lacking USP50, or FEN1 are driven by these proteins. Consequently, suppression of DNA2 or RECQL4/5 improves USP50-depleted cell resistance to agents inducing replicative stress and restores telomere stability. These data define an unexpected regulatory protein that promotes the balance of helicase and nuclease use at ongoing and stalled replication forks..

2024-08-21 | GSE269605 | GEO

USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication.

Project description:Mammalian DNA replication relies on various DNA helicase and nuclease activities to ensure accurate genetic duplication, but how different helicase and nuclease activities are properly directed remains unclear. Here, we identify the ubiquitin-specific protease, USP50, as a chromatin-associated protein required to promote ongoing replication, fork restart, telomere maintenance, cellular survival following hydroxyurea or pyridostatin treatment, and suppression of DNA breaks near GC-rich sequences. We find that USP50 supports proper WRN-FEN1 localisation at or near stalled replication forks. Nascent DNA in cells lacking USP50 shows increased association of the DNA2 nuclease and RECQL4 and RECQL5 helicases and replication defects in cells lacking USP50, or FEN1 are driven by these proteins. Consequently, suppression of DNA2 or RECQL4/5 improves USP50-depleted cell resistance to agents inducing replicative stress and restores telomere stability. These data define an unexpected regulatory protein that promotes the balance of helicase and nuclease use at ongoing and stalled replication forks.

| S-EPMC11405836 | biostudies-literature

A whole genome-screening strategy to identify DNA2 binding sites and genomic regions that require DNA2 for replication

Project description:We report that DNA2 predominantly bound to the centromeric α-satellite regions. The centromeric regions contained 58% of the DNA2-associated DNA, representing a 33.5-fold enrichment over genomic input. To define the under-replicated DNA regions in DNA2-null cells, we conducted whole-genome DNA sequencing of the under-replicated BrdU negative DNA. After normalization to genomic input DNA from the same cells, 12.9% of the peaks from the under-replicated DNA aligned with the centromeric DNA regions, representing an 8.5-fold enrichment. In addition, among the peaks that overlapped between the DNA2 pull-down and under-replicated regions, two thirds fell into the centromeric regions, representing a 48-fold enrichment.

2018-04-10 | GSE108619 | GEO

DNA2

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DNA2

Project description:Sus scrofa DNA2, DNA replication helicase/nuclease 2 [Source:VGNC Symbol;Acc:VGNC:87361], is differentially expressed in 1 experiment(s);

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DNA2

Project description:Sus scrofa DNA2, DNA replication helicase/nuclease 2 [Source:VGNC Symbol;Acc:VGNC:87361], is expressed in 2 baseline experiment(s);

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Project description:Macaca mulatta DNA2, DNA replication helicase/nuclease 2 [Source:HGNC Symbol;Acc:HGNC:2939], is expressed in 2 baseline experiment(s);

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DNA2

Project description:Homo sapiens DNA2, DNA replication helicase/nuclease 2 [Source:HGNC Symbol;Acc:HGNC:2939], is differentially expressed in 172 experiment(s);

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Dna2

Project description:Mus musculus Dna2, DNA replication helicase/nuclease 2 [Source:MGI Symbol;Acc:MGI:2443732], is differentially expressed in 152 experiment(s);

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