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Dissociation of mitochondrial and ribosomal biogenesis during thallium administration in rat kidney

ABSTRACT: Dissociation of mitochondrial and ribosomal biogenesis during thallium administration in rat kidney

PROVIDER: PRJNA1122847 | ENA |

REPOSITORIES: ENA

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Dissociation of mitochondrial and ribosomal biogenesis during thallium administration in rat kidney

Project description:Total RNAs from the control and thallium groups were subjected to DNA microarray analysis. Transcriptome analysis showed that many of the genes were upregulated by Tl administration.

2024-08-07 | GSE269635 | GEO

Dissociation of mitochondrial and ribosomal biogenesis during thallium administration in rat kidney.

Project description:Thallium (Tl) is a heavy metal with toxicity comparative to other heavy metals such as As, Cd, and Hg. Nevertheless, fewer studies have been reported concerning the molecular mechanism of Tl toxicity as compared to other heavy metals. To obtain insight into Tl toxicity in the kidney, rats were intraperitoneally administered Tl2SO4 (30 mg/kg), and the kidneys were removed 2 or 5 days later to examine the effects of Tl. Transcriptome analysis using DNA microarray of the rat kidney 2 and 5 days after Tl administration showed that cytoplasmic ribosomal proteins are the most upregulated category; many of the genes involved in ribosome biosynthesis were upregulated by Tl administration. This upregulation was associated with the activation of eukaryotic transcription initiation factor 2α (eIF2α), implying that increased ribosome biogenesis was linked to the subsequent activation of protein translation. In contrast, decreased mitochondrial biogenesis was revealed via proteomic analysis. Although we found an increase in Myc, a positive regulator of both ribosomal and mitochondrial biogenesis, decreased levels of NRF1 and TFAM, positive regulators of mitochondrial biogenesis whose gene expression is directory activated by Myc, were paradoxically observed. Taken together, differing responses of ribosomes and mitochondria to Tl toxicity were observed. Failure of transmission of the Myc signal to NRF1/TFAM might be involved in the observed disruption of coordinated responses in mitochondria and ribosomes during Tl administration in rat kidney.

| S-EPMC11616847 | biostudies-literature

The roles of GTPases in biogenesis of the large ribosomal subunit

Project description:Ribosomes are ribonucleoprotein complexes responsible for protein synthesis, and their biogenesis is a complex, highly orchestrated process. Formation of the ribosomal subunits is coordinated by trans-acting ribosome biogenesis factors (RBFs) that mediate ribosomal RNA (rRNA) modification, folding and processing, as well as the incorporation and positioning of ribosomal proteins (RPs). GTPases are among the RBFs that consume energy to drive structural rearrangements of pre-ribosomal particles, and several of these enzymes play important roles in assembly of the large subunit. Protein-RNA crosslinking approaches were used to identify GNL3L binding sites on pre-ribosomes and map RNA contact sites on the GTPase. The findings of this study support the involvement of GNL3L in large subunit biogenesis and provide new insights into its functions in this process.

2026-03-11 | GSE302147 | GEO

Puf3 participates in ribosomal biogenesis in malaria parasites

Project description:Puf3 participates in ribosomal biogenesis in malaria parasites

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Exonuclease Requirements for Mammalian Ribosomal RNA Biogenesis and Surveillance

Project description:Exonuclease Requirements for Mammalian Ribosomal RNA Biogenesis and Surveillance

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7q11.23 CNV alters ribosomal biogenesis, mTOR and neuronal intrinsic excitability

Project description:7q11.23 CNV alters ribosomal biogenesis, mTOR and neuronal intrinsic excitability

| PRJNA1088432 | ENA

The Timing of Ribosomal RNA Modification During Eukaryotic Ribosome Biogenesis

Project description:The Timing of Ribosomal RNA Modification During Eukaryotic Ribosome Biogenesis

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The Timing of Ribosomal RNA Modification During Eukaryotic Ribosome Biogenesis

Project description:The Timing of Ribosomal RNA Modification During Eukaryotic Ribosome Biogenesis

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The Timing of Ribosomal RNA Modification During Eukaryotic Ribosome Biogenesis

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Ribosomal mistranslation leads to silencing of the unfolded protein response and increased mitochondrial biogenesis

Project description:Translation fidelity is the limiting factor in the accuracy of gene expression. With an estimated frequency of 10-4, errors in mRNA decoding occur in a mostly stochastic manner. Little is known about the response of higher eukaryotes to chronic loss of ribosomal accuracy as per an increase in the random error rate of mRNA decoding. Here, we present a global and comprehensive picture of the cellular changes in response to translational accuracy in mammalian ribosomes impaired by genetic manipulation. In addition to affecting established protein quality control pathways, such as elevated transcript levels for cytosolic chaperones, activation of the ubiquitin-proteasome system, and translational slowdown, ribosomal mistranslation led to unexpected responses. In particular, we observed increased mitochondrial biogenesis associated with import of misfolded proteins into the mitochondria and silencing of the unfolded protein response in the endoplasmic reticulum. This study describes the metabolomic analysis of HEK293 cells lines expressing mutant ribosomal protein RPS2 (human A226Y). RPS2 A226Y mutation has been shown to cause misreading and readthrough. Results provide insight into the response to chronic mistranslation in mammalian cells.

2019-09-17 | MTBLS106 | MetaboLights

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