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Heterogeneous macrophage activation in injured skeletal muscle

ABSTRACT: Heterogeneous macrophage activation in injured skeletal muscle

PROVIDER: PRJNA1124889 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29433644.fastq.gz	Fastqsanger.gz
	SRR29433645.fastq.gz	Fastqsanger.gz
	SRR29433646.fastq.gz	Fastqsanger.gz
	SRR29433647.fastq.gz	Fastqsanger.gz
	SRR29433648.fastq.gz	Fastqsanger.gz

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Heterogeneous macrophage activation in injured skeletal muscle

Project description:Macrophages are the predominant inflammatory cells during skeletal muscle injury repair. They are required for the complete repair of acutely injured skeletal muscle, but detrimentally contribute to the pathological progression of dystrophic muscle in Duchenne muscular dystrophy (DMD) patients and DMD mouse model mdx5cv mice. The cellular and molecular mechanism underlying this difference has yet to be fully elucidated. To this end, the present study compared the single-cell transcriptomes of intramuscular monocytes/macrophages from uninjured and acutely injured quadriceps of 14 weeks-old wild-typed (WT) mice, and from quadriceps and diaphragm of age-matched mdx5cv mice, using single cell-based RNA sequencing (scRNA-seq) analysis. Our results identified multiple functionally diverse monocyte/macrophage clusters co-existing in each injured muscle sample. Both Ly6Chi and Ly6Clo intramuscular monocytes/macrophages were heterogenous, containing different clusters among different injury conditions. These clusters did not conform to strict M1/M2 activation, and were involved differentially in inflammation, extracellular matrix (ECM) remodeling, and myogenesis during skeletal muscle injury repair. These findings uncovered the heterogeneity of monocyte/macrophage activation in both acutely injured and dystrophic skeletal muscle, shedding light on the molecular and cellular mechanisms underlying the differential roles of monocytes/macrophages in acute skeletal muscle injury and muscular dystrophy in DMD.

2025-06-14 | GSE270031 | GEO

Regulatory T cells from injured skeletal muscle

Project description:A phenotypically and functionally distinct population of CD4+ Foxp3+ T cells (Tregs) rapidly accumulates in acutely injured skeletal muscle of mice, just as invading myeloid-lineage cells switch from a pro-inflammatory to a pro-regenerative state. Analysis of gene expression of Tregs and CD4+Foxp3- T cells (Tconvs) from injured muscle and spleen revealed that the transcriptome of muscle Treg cells is distinct from that of splenic Tregs. A set of genes is uniquely expressed by muscle Tregs, while another set is over-expressed by the two muscle populations vis-à-vis their two spleen counterparts. 6 wk-old Foxp3-ires-GFP mice were injured in skeletal muscles with cardiotoxin. Four and fourteen days later, Tregs and Tconvs from spleen and muscle were double-sorted into Trizol. To reduce variability, cells from multiple mice were pooled for sorting, and three replicates were generated for all groups. RNA from 1.5-2.5 x 104 cells was amplified, labeled, and hybridized to Affymetrix Mouse Gene 1.0 ST Arrays.

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Cripto shapes macrophage plasticity and restricts EndMT in injured and diseased skeletal muscle

Project description:Cripto shapes macrophage plasticity and restricts EndMT in injured and diseased skeletal muscle

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Cripto shapes macrophage plasticity and restricts EndMT in injured and diseased skeletal muscle

Project description:The membrane protein Cripto plays a key role in shaping macrophage plasticity in skeletal muscle during regeneration and disease. Cripto acts as an extrinsic modulator of macrophage plasticity and is required for the proper expansion/maintenance of the CD206+ anti-inflammatory macrophage population. Nevertheless, Cripto deletion does not change the gene expression profile of F4/80+/Ly6CLow macrophages suggesting that Cripto was dispensable to induce/maintain the Ly6CLow phenotype.

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Regulatory T cells from injured skeletal muscle

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Treg cells limit IFN-g production to control macrophage accrual and phenotype during skeletal muscle regeneration

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Gene expression profiling of injured skeletal muscle in the presence or absence of CD4+Foxp3+ regulatory T cells (Tregs).

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Heterogeneous Origins and Functions of Mouse Skeletal Muscle Resident Macrophages

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