Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [HRH1]

ABSTRACT: Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [HRH1]

PROVIDER: PRJNA1124957 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [FZD4]

Project description:Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [FZD4]

| PRJNA1124958 | ENA

Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [ACKR3]

Project description:Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [ACKR3]

| PRJNA1124955 | ENA

Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [AODRA1]

Project description:Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [AODRA1]

| PRJNA1124959 | ENA

Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [HRH1]

Project description:Human U2OS cells were stimulated with selective agonist PEA of Histamine receptor H1 (HRH1) for 24 hours. Extracellular vesicles were isolated from U2OS cell culture media. Total RNAs were purified from extracellular vesicle samples. We used TaqMan Low Density Arrays to quantitate miRNA expression in extracellular vesicles secreted by U2OS cells after GPCR activation.

2025-07-30 | GSE270080 | GEO

Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [FZD4]

Project description:Human U2OS cells were stimulated with selective agonist Wnt3a of Frizzled class receptor 4(FZD4) for 24 hours. Extracellular vesicles were isolated from U2OS cell culture media. Total RNAs were purified from extracellular vesicle samples. We used TaqMan Low Density Arrays to quantitate miRNA expression in extracellular vesicles secreted by U2OS cells after GPCR activation.

2025-07-30 | GSE270079 | GEO

Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [AODRA1]

Project description:Human U2OS cells were stimulated with selective agonist CCPA of adenosine receptor A1(ADORA1) for 24 hours. Extracellular vesicles were isolated from U2OS cell culture media. Total RNAs were purified from extracellular vesicle samples. We used TaqMan Low Density Arrays to quantitate miRNA expression in extracellular vesicles secreted by U2OS cells after GPCR activation.

2025-07-30 | GSE270078 | GEO

Real-time quantitative PCR analysis of extracellular vesicle miRNA from human U2OS cells culture medium [ACKR3]

Project description:Human U2OS cells were stimulated with selective agonist SDF-1 of atypical chemokine receptor 3 (ACKR3) for 24 hours. Extracellular vesicles were isolated from U2OS cell culture media. Total RNAs were purified from extracellular vesicle samples. We used TaqMan Low Density Arrays to quantitate miRNA expression in extracellular vesicles secreted by U2OS cells after GPCR activation.

2025-07-30 | GSE270077 | GEO

Comparison of LC-MS/MS and real-time PCR assays for the efficient detection of potentially allergenic silkworm

Project description:The silkworm (Bombyx mori) has long been considered a source of food and medicine due to its high nutritional, medicinal, and economic value in East Asia. However, in some sensitive individuals, silkworm consumption can cause allergenic reactions such as vomiting, asthma, and anaphylaxis. Therefore, the development of a reliable method for silkworm detection is required to avoid such allergenic incidents. In this study, two different methods (liquid chromatography combined with mass spectrometry [LC-MS/MS] and real-time polymerase chain reaction [PCR]) were developed to determine an efficient technique for silkworm detection in foods. The developed methods demonstrated high sensitivity in detecting the silkworm in processed foods. Silkworm-spiked model cookies were used to confirm the sensitivity of both LC-MS/MS (0.0005%) and real-time PCR (0.001%). These methods were found to be useful for detecting the silkworm in foods and avoiding allergenic reactions. To the best of our knowledge, this is the first study to compare LC-MS/MS and real-time PCR for silkworm detection in complex processed foods.

2024-05-24 | PXD042494 | Pride

Real-time quantitative PCR analysis of human exosomes

Project description:Real-time quantitative PCR analysis of human exosomes

| PRJNA504728 | ENA

Real-time quantitative PCR analysis of human serum

Project description:Real-time quantitative PCR analysis of human serum

| PRJNA714377 | ENA