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ABSTRACT: Quinoa seed Transcriptome

PROVIDER: PRJNA1124963 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29436800_1.fastq.gz	Fastqsanger.gz
	SRR29436800_2.fastq.gz	Fastqsanger.gz
	SRR29436801_1.fastq.gz	Fastqsanger.gz
	SRR29436801_2.fastq.gz	Fastqsanger.gz
	SRR29436802_1.fastq.gz	Fastqsanger.gz

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Chenopodium quinoa

Project description:Quinoa seed Transcriptome

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Project description:Quinoa seed Transcriptome

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TMT proteomic analysis of tropical quinoa (Chenopodium quinoa willd.) seeds during maturation

Project description:Many limitation factors, such as seed pre-harvest sprouting, bitter saponin e.c., greatly restricts quinoa production and popularity. To solves these problems, the underlying mechanism of seed maturation in quinoa was required. In this study, morphological observation, TMT proteomics and parallel reaction monitoring (PRM) were conducted to investigate the variation of quinoa seed during seed maturation

2022-10-19 | PXD034885 | Pride

The diversity of quinoa morphological traits and seed metabolic composition

Project description:<p>Quinoa (<em>Chenopodium quinoa</em> Willd.) is an herbaceous annual crop of the amaranth family (Amaranthaceae). It is increasingly cultivated for its nutritious grains, which are rich in protein and essential amino acids, lipids, and minerals. Quinoa exhibits a high tolerance towards various abiotic stresses including drought and salinity, which supports its agricultural cultivation under climate change conditions. The use of quinoa grains is compromised by anti-nutritional saponins, a terpenoid class of secondary metabolites deposited in the seed coat; their removal before consumption requires extensive washing, an economically and environmentally unfavorable process; or their accumulation can be reduced through breeding. In this study, we analyzed the seed metabolomes, including amino acids, fatty acids, and saponins, from 471 quinoa cultivars, including two related species, by liquid chromatography - mass spectrometry. Additionally, we determined a large number of agronomic traits including biomass, flowering time, and seed yield. The results revealed considerable diversity between genotypes and provide a knowledge base for future breeding or genome editing of quinoa.</p>

2022-03-10 | MTBLS2382 | MetaboLights

Quinoa seed proteome - Characterization of quinoa seed proteome combining different protein precipitation techniques: Improvement of knowledge of nonmodel plant proteomics

Project description:A shotgun proteomics approach was used to characterize the quinoa seed proteome. To obtain comprehensive proteomic data from quinoa seeds three different precipitation procedures were employed: MeOH/CHCl3/ddH2O, acetone either alone or with trichloroacetic acid; the isolated proteins were then in-solution digested and the resulting peptides analyzed bynano liquid chromatography coupled to tandem mass spectrometry. However, since quinoa is a non model plant species, only a few protein sequences are included in the most widely known protein sequence databases. To improve the data reliability a UniProt subdatabase, containing only proteins of Caryophillales order, was used.

2016-06-24 | PXD001631 | Pride

RNAseq of Quinoa salt bladders vs. leaves

Project description:Chenopodium quinoa, a pseudo-cereal and facultative halophyte, is a species of great economic potential. When exposed to saline soil, this salt-tolerant crop takes up sodium and chloride ions and sequesters large NaCl quantities in epidermal bladders cells (EBC). We have analyzed the Quinoa EBC transcriptome by RNA sequencing and elucidated the molecular identity and function of key ion transporters. Thereby we analyzed transcripts differentially expressed between EBCs and total leaves under control conditions.

2018-09-20 | E-MTAB-6112 | biostudies-arrayexpress

Next-Generation Sequencing Facilitates Quantitative Analysis of Two Kinds of Chenopodium quinoa Transcriptomes

Project description:We generated 95.37 Gb of high-quality sequencing data (~7.95 Gb per sample). The analysis showed differences of transcriptomes between the common white sweet quinoa and the yellow bitter quinoa. We identified numerous differentially expressed genes that exhibited distinct expression patterns. These genes have known or potential roles in taste of quinoa fruit.Therefore, we are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to the accumulation of bitter saponins in C. quinoa fruits.

2021-09-17 | GSE139174 | GEO

Genome-wide analysis of CqCrRLK1Ls and CqRALFs in Chenopodium quinoa

Project description:Quinoa (Chenopodium quinoa) is a highly nutritious crop showing a remarkable tolerance to multiple abiotic stresses. The Catharanthus roseus RLK1-like kinase (CrRLK1L) family is involved in multiple processes during plant growth and stress responses. However, little is known about the members of the CqCrRLK1L subfamily and their biological functions. In this study, up to 26 CqCrRLK1L members were identified in quinoa genome. To systematically investigate their tissue-specific expression pattern and gene expression profiles in response to abiotic stresses, different quinoa samples including leaf, pistil, stamen, root, and leaf treated with NaCl were harvested for transcrioptome analysis with three biological replicates for each sample. Finally,we mapped about 30 million sequence reads per sample to the quinoa genome (NL-6) and combining the four different tissues (leaf, pistil, stamen, and root), totally 40382 genes were detected, accounting for 69% of total genes in quinoa. The expression of most CqCrRLK1Ls genes were detected in all four kind of tissues, and they showed tissue-specific expression pattern. In salt-treated leaves, we observe that about 6-thosands genes changed their expression, with log2FoldChange < -1 or >1 and p value <0.05. And four CqCrRLK1L genes largely altered their transcript levels, implying that these genes are involved in the regulation of salt stress response in quinoa. The present study provides a base for future research on elucidating the varied biological functions of CqCrRLK1Ls and their contributions to stress responses.

2022-06-27 | GSE198572 | GEO

Project description:Genetic basis of seed colour in quinoa

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Chenopodium quinoa

Project description:Comparative transcriptomic analyses of quinoa (Chenopodium quinoa) seed accessions from diverse geographic locations

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