Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA112619 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The development of trichomes (leaf hairs) from pluripotent epidermal cells in Arabidopsis provides a powerful system to investigate the regulatory motifs involved in plant cell differentiation. Genetic studies have revealed that a bHLH transcription factor, GL3, activates downstream genes required for trichome initiation by interacting with a R2R3-MYB protein, GL1. In order to investigate genome-wide regulatory functions of GL1 and GL3, we performed genome-wide expression analyses using GR inducible systems of GL1 and GL3.
2009-03-02 | GSE12551 | GEO
Project description:GLABRA3 (GL3) and GLABRA1 (GL1) function as selector genes that control the differentiation of a group of protodermal cells into trichomes in Arabidopsis thaliana. We performed genome-wide location (ChIP-chip) analyses by using transgenic Arabidopsis plants carrying GL3-YFP or GL1-YFP-MYC1 mini genes. These analyses identified statistically significant enriched DNA associated with GL1 and GL3. A total ~540 and ~700 genes were identified as located proximal and downstream to the GL1 and GL3 binding regions, respectively. Keywords: ChIP-chip
2009-03-02 | GSE13090 | GEO
Project description:The development of trichomes (leaf hairs) from pluripotent epidermal cells in Arabidopsis provides a powerful system to investigate the regulatory motifs involved in plant cell differentiation. Genetic studies have revealed that a bHLH transcription factor, GL3, activates downstream genes required for trichome initiation by interacting with a R2R3-MYB protein, GL1. In order to investigate genome-wide regulatory functions of GL1 and GL3, we performed genome-wide expression analyses using GR inducible systems of GL1 and GL3. Transgenic plants carrying pGL1::GL1-GR and pGL3::GL3-GR were treated with mock or Dex for 4 and 24 hours.
2009-03-02 | E-GEOD-12551 | biostudies-arrayexpress
Project description:The development of trichomes (leaf hairs) from pluripotent epidermal cells in Arabidopsis provides a powerful system to investigate the regulatory motifs involved in plant cell differentiation. Genetic studies have revealed that a bHLH transcription factor, GL3, activates downstream genes required for trichome initiation by interacting with a R2R3-MYB protein, GL1. We have taken advantage of several mutants in the trichome developmental pathway and gene expression analyses to identify a set of genes expressed predominantly in Arabidopsis trichomes. Experiment Overall Design: We compared the gene expression between the wild type and trichome mutant, gl3 egl3 using green tissue (not include root).
2009-03-07 | E-GEOD-12522 | biostudies-arrayexpress
Project description:The development of trichomes (leaf hairs) from pluripotent epidermal cells in Arabidopsis provides a powerful system to investigate the regulatory motifs involved in plant cell differentiation. Genetic studies have revealed that a bHLH transcription factor, GL3, activates downstream genes required for trichome initiation by interacting with a R2R3-MYB protein, GL1. We have taken advantage of several mutants in the trichome developmental pathway and gene expression analyses to identify a set of genes expressed predominantly in Arabidopsis trichomes.
2009-03-02 | GSE12522 | GEO
Project description:Genome-wide GL3 and GL1 binding sites in Arabidopsis thaliana
| PRJNA110915 | ENA
Project description:Trichomes contribute to plant tolerance of abiotic stresses, such as heat, and biotic stresses, including insect herbivory. Leaves of Brassica napus are typically glabrous or near-glabrous; however, a small number of hirsute accessions were identified in international germplasm collections that produced up to 300 trichomes on the earliest leaves. To investigate the genetic basis of this trait, trichome density on the abaxial and adaxial leaf surfaces, leaf margins, and petioles of the 3rd and 6th leaves was assessed in a doubled haploid (DH) population derived from a cross between a glabrous line (NAM-0) and a hirsute line (DOS-2). Lines were genotyped using a Brassica 60K SNP BeadChip array. A major quantitative trait locus (QTL) controlling trichome density was identified on chromosome C01 that overlapped with regions previously mapped in the C-genome species B. oleracea and B. villosa. In B. napus, this QTL spanned 5.65 Mb and with a 1.7 Mb deletion in the hirsute DOS-2 parent. A gene encoding a novel, R3 single-repeat, MYB protein related to the trichome repressor TRIPTYCHON was identified within the shared region and designated TRIPTYCHON-LIKE (TRL). DOS-2 harbors a single BnTRL gene, whereas NAM-0 contains four tandemly-linked copies, although BnTRL transcript abundance did not correlate directly with trichome density. The expanded TRL gene family is unique to the Brassicaceae and BnTRL proteins physically interact with the GL1-binding domain of GL3, a core component of the GL1–GL3–TTG1 trichome initiation complex. Analysis of additional genes within the shared QTL region or absent in DOS-2 revealed regulators associated with chromatin modification (e.g. ADA2b, RBBP6-like), hormone signaling (ARR2, GH3, JAM3, HAT4), cytoskeletal dynamics (SPIRAL2, MAP65-2, MAP70-5, SPIKE1), and cell growth (SVH3, LIP5). Hormone application experiments demonstrated genotype- and tissue-specific responses on trichome production, particularly to jasmonate and gibberellin. Together, these results indicate that the CO1 locus controlling trichome abundance in B. napus is complicated and may affect regulation of chromatin state, hormonal pathways, cytoskeletal organization, and cell growth, in addition to the assembly of the trichome regulatory complex.
2026-03-17 | GSE324722 | GEO
Project description:Transcriptional profiling of isolated gl3-sst trichome cells.
2009-02-03 | E-MEXP-2009 | biostudies-arrayexpress
Project description:Trichomes of Arabidopsis thaliana have been broadly used to study cell development, cell differentiation and cell wall biogenesis. In this context, the exposed position, extraordinary size and characteristic morphology of trichomes featured particularly the exploration of trichome mutant phenotypes. However, trichome-specific biochemical or -omics analyses require a proper separation of trichomes from residual plant tissue. Thus, different strategies were proposed in the past for trichome isolation, which mostly rely on harsh conditions. To improve trichome-leaf separation, we revised a previously proposed method for isolating A. thaliana trichomes by optimizing the mechanical and biochemical specifications for trichome release. Furthermore, we introduced a density gradient centrifugation step to remove residual plant debris. We found that prolonged, yet mild seedling agitation increases the overall trichome yield by about 62% compared to the original protocol. We noticed that subsequent density gradient centrifugation further visually enhances trichome purity, which could be advantageous for downstream analyses. Histochemical and biochemical investigation of trichome cell wall composition indicated that gentle agitation during trichome release largely preserves trichome integrity. We used enriched and purified trichomes for proteomic analysis and present a reference data set of trichome-resident and -enriched proteins.
2022-01-13 | PXD028808 | Pride
Project description:Comparison of gene expression between wild type and gl3 egl3 trichomeless mutant green seedling tissues
| PRJNA112923 | ENA