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Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton

ABSTRACT: Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton

PROVIDER: PRJNA1127543 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29529345_1.fastq.gz	Fastqsanger.gz
	SRR29529345_2.fastq.gz	Fastqsanger.gz
	SRR29529346_1.fastq.gz	Fastqsanger.gz
	SRR29529346_2.fastq.gz	Fastqsanger.gz
	SRR29529347_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton

Project description:Xanthomonas spp. employ transcription activator-like effectors (TALEs) to promote pathogenicity by activating host susceptibility (S) genes. Cotton GhSWEET10 is an S gene targeted by a TALE in an early isolate of Xanthomonas citri pv. malvacearum (Xcm), but not by recent field Xcm isolates. To understand the pathogenicity shift in Xcm and its adaptation to cotton, we assembled the whole genome and the TALE repertoire of three recent Xcm Texas field isolates. A newly evolved TALE, Tal7b, activated different GhSWEET genes, GhSWEET14a and GhSWEET14b. Simultaneous activation of GhSWEET14a and GhSWEET14b resulted in pronounced water-soaked lesions. Transcriptome profiling coupled with TALE-binding element prediction identified a pectin lyase as an additional Tal7b target, quantitatively contributing to Xcm virulence alongside GhSWEET14a/b. CRISPR-Cas9-based gene editing supported the function of GhSWEETs as S genes in cotton bacterial blight and the promise of disrupting the TALE-binding site in these genes to control the disease. Collectively, our findings elucidate the rapid evolution of TALEs in Xanthomonas field isolates and highlight the virulence mechanism wherein TALEs induce multiple S genes simultaneously to promote pathogenicity.

2024-06-26 | GSE270600 | GEO

Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton.

Project description:Bacterial transcription activator-like effectors (TALEs) promote pathogenicity by activating host susceptibility (S) genes. To understand the pathogenicity and host adaptation of Xanthomonas citri pv. malvacearum (Xcm), we assemble the genome and the TALE repertoire of three recent Xcm Texas isolates. A newly evolved TALE, Tal7b, activates GhSWEET14a and GhSWEET14b, different from GhSWEET10 targeted by a TALE in an early Xcm isolate. Activation of GhSWEET14a and GhSWEET14b results in water-soaked lesions. Transcriptome profiling coupled with TALE-binding element prediction identify a pectin lyase gene as an additional Tal7b target, quantitatively contributing to Xcm virulence alongside GhSWEET14a/b. CRISPR-Cas9 gene editing supports the function of GhSWEETs in cotton bacterial blight and the promise of disrupting the TALE-binding site in S genes for disease management. Collectively, our findings elucidate the rapid evolution of TALEs in Xanthomonas field isolates and highlight the virulence mechanism wherein TALEs induce multiple S genes to promote pathogenicity.

| S-EPMC11733179 | biostudies-literature

TAL effector driven induction of a SWEET gene confers susceptibility to bacterial blight of cotton.

Project description:Transcription activator-like (TAL) effectors from Xanthomonas citri subsp. malvacearum (Xcm) are essential for bacterial blight of cotton (BBC). Here, by combining transcriptome profiling with TAL effector-binding element (EBE) prediction, we show that GhSWEET10, encoding a functional sucrose transporter, is induced by Avrb6, a TAL effector determining Xcm pathogenicity. Activation of GhSWEET10 by designer TAL effectors (dTALEs) restores virulence of Xcm avrb6 deletion strains, whereas silencing of GhSWEET10 compromises cotton susceptibility to infections. A BBC-resistant line carrying an unknown recessive b6 gene bears the same EBE as the susceptible line, but Avrb6-mediated induction of GhSWEET10 is reduced, suggesting a unique mechanism underlying b6-mediated resistance. We show via an extensive survey of GhSWEET transcriptional responsiveness to different Xcm field isolates that additional GhSWEETs may also be involved in BBC. These findings advance our understanding of the disease and resistance in cotton and may facilitate the development cotton with improved resistance to BBC.

| S-EPMC5458083 | biostudies-literature

Genomics-enabled analysis of the emergent disease cotton bacterial blight

Project description:Cotton bacterial blight (CBB), an important disease of (Gossypium hirsutum) in the early 20th century, had been controlled by resistant germplasm for over half a century. Recently, CBB re-emerged as an agronomic problem in the United States. Analysis of cotton variety planting statistics indicates a steady increase in the percentage of susceptible cotton varieties grown each year since 2009. Phylogenetic analysis revealed that strains from the current outbreak cluster with race 18 Xanthomonas citri pv. malvacearum (Xcm) strains. Illumina based draft genomes were generated for thirteen Xcm isolates. These genomes, along with 4 previously published Xcm genomes, encode 24 conserved and nine variable type three effectors. Strains in the race 18 clade contain 3 to 5 more effectors than other Xcm strains. SMRT sequencing of two geographically and temporally diverse strains of Xcm yielded circular chromosomes and accompanying plasmids. These genomes encode eight and thirteen distinct transcription activator-like effector genes. RNA-sequencing revealed 52 genes induced within two cotton cultivars by both tested Xcm strains. This gene list includes a homeologous pair of genes, with homology to the known susceptibility gene, MLO. In contrast, the two strains of Xcm induce different class III SWEET sugar transporters. Subsequent genome wide analysis revealed patterns in the overall expression of homeologous gene pairs in cotton after inoculation by Xcm. These data reveal host-pathogen specificity at the genetic level and strategies for future development of resistant cultivars.

2017-07-24 | GSE101778 | GEO

The Xa7 Resistance Gene Guards the Susceptibility Gene SWEET14 of Rice Against Exploitation by Bacterial Blight Pathogen

Project description:TAL effectors of bacteria hijack host gene expression to condition disease susceptibility, while host plants counteract TAL effectors with resistance genes to thwart pathogen infections. Here we report the cloning of Xa7, TAL effector-specific expression by the essential virulence TAL effectors AvrXa7 and PthXo3, and the prevalence of Xa7 in indica rice cultivars.

2021-01-27 | GSE165583 | GEO

Resistant response of leaves of upland cotton line Im216 to Xanthomonas campestris pv. malvacearum race 1

Project description:Gene expression profiling of upland cotton line Im216 to inoculation with Xanthomonas campestris pv. malvacearum race 1. Fifth or sixth leaves of the bacterial blight-resistant cotton line Im216, which had been grown in a plant growth chamber, were infiltrated with a suspension of about 5x10^6 colony-forming units ml^-1 of Xanthomonas campestris pv. malvacearum race 1 in sterile saturated CaCO3 solution or were not inoculated (control). Keywords: Time-course

2006-01-01 | GSE2851 | GEO

Xanthomonas : TAL mutant of common bacterial blight of bean : 6165R(delta)tal22B and 6546R(delta)tal18H

Project description:TAL mutant of common bacterial blight of bean agents : 6165R(delta)tal22B and 6546R(delta)tal18H

| PRJNA715109 | ENA

Code-assisted discovery of TAL effector targets in bacterial leaf streak of rice reveals contrast with bacterial blight and a novel susceptibility gene.

Project description:Bacterial leaf streak of rice, caused by Xanthomonas oryzae pv. oryzicola (Xoc) is an increasingly important yield constraint in this staple crop. A mesophyll colonizer, Xoc differs from X. oryzae pv. oryzae (Xoo), which invades xylem to cause bacterial blight of rice. Both produce multiple distinct TAL effectors, type III-delivered proteins that transactivate effector-specific host genes. A TAL effector finds its target(s) via a partially degenerate code whereby the modular effector amino acid sequence identifies nucleotide sequences to which the protein binds. Virulence contributions of some Xoo TAL effectors have been shown, and their relevant targets, susceptibility (S) genes, identified, but the role of TAL effectors in leaf streak is uncharacterized. We used host transcript profiling to compare leaf streak to blight and to probe functions of Xoc TAL effectors. We found that Xoc and Xoo induce almost completely different host transcriptional changes. Roughly one in three genes upregulated by the pathogens is preceded by a candidate TAL effector binding element. Experimental analysis of the 44 such genes predicted to be Xoc TAL effector targets verified nearly half, and identified most others as false predictions. None of the Xoc targets is a known bacterial blight S gene. Mutational analysis revealed that Tal2g, which activates two genes, contributes to lesion expansion and bacterial exudation. Use of designer TAL effectors discriminated a sulfate transporter gene as the S gene. Across all targets, basal expression tended to be higher than genome-average, and induction moderate. Finally, machine learning applied to real vs. falsely predicted targets yielded a classifier that recalled 92% of the real targets with 88% precision, providing a tool for better target prediction in the future. Our study expands the number of known TAL effector targets, identifies a new class of S gene, and improves our ability to predict functional targeting.

| S-EPMC3937315 | biostudies-literature

Correction: Code-Assisted Discovery of TAL Effector Targets in Bacterial Leaf Streak of Rice Reveals Contrast with Bacterial Blight and a Novel Susceptibility Gene

Project description: Not available

| S-EPMC3989325 | biostudies-literature

A structurally dynamic chromosome 11 locus from Oryza rufipogon L. confers broad-spectrum recessive resistance to bacterial blight

Project description:Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most destructive diseases affecting rice production worldwide. In this study, the wild rice accession Oryza rufipogon Wild173 (UP36) exhibiting strong resistance to multiple Xoo strains was investigated in comparison with the susceptible rice cultivar Longjing-31 (CK2). To explore the molecular mechanisms underlying bacterial blight resistance, transcriptome sequencing (RNA-seq) was performed on leaf tissues collected from resistant and susceptible plants following inoculation with the Xoo strain PXO99A. Comparative transcriptomic analysis was conducted to identify differentially expressed genes associated with defense responses. The dataset provides insights into transcriptional reprogramming during pathogen infection and facilitates the identification of candidate genes and defense-related pathways involved in bacterial blight resistance in rice. These data provide valuable resources for understanding host–pathogen interactions and for improving disease resistance in rice breeding programs.

2026-03-14 | GSE324324 | GEO

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