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Dnmt1 determines bone length by regulating energy metabolism of growth plate chondrocytes [RNA-seq]

ABSTRACT: Dnmt1 determines bone length by regulating energy metabolism of growth plate chondrocytes [RNA-seq]

PROVIDER: PRJNA1127613 | ENA |

REPOSITORIES: ENA

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Dnmt1 determines bone length by regulating energy metabolism of growth plate chondrocytes [MBD-seq]

Project description:Chondrocytes differentiated from mesenchymal stem cells play a role in determining skeletal patterns by ossification. However, it is still unclear how the maintenance DNA methylation in chondrocytes regulates differentiation and skeletal formation. In Musculoskeletal Knowledge Portal, Dnmt1 was significantly associated with “Height”. In fact, long bone of limb specific Dnmt1 deficient mice was significantly shortened due to decreased chondrocyte proliferation and accelerated differentiation. Furthermore, integrated analysis of RNA-Seq and MBD-Seq revealed that in Dnmt1ΔPrx1 chondrocytes, reduced DNA methylation resulted in an increase in energy metabolism-related genes in addition to ossification-related genes. Metabolomic analysis confirmed that almost all energy metabolites were increased in Dnmt1ΔPrx1 chondrocytes. These results indicate that Dnmt1 mediated maintenance DNA methylation governs chondrocyte differentiation by regulating energy metabolism through both gene expression and metabolite supply. Taken together, this study suggested that appropriate DNA methylation status in chondrocytes can orchestrate growth plate mineralization and subsequently determine bone length.

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Dnmt1 determines bone length by regulating energy metabolism of growth plate chondrocytes [RNA-seq]

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Dnmt1 determines bone length by regulating energy metabolism of growth plate chondrocytes [MBD-seq]

Project description:Dnmt1 determines bone length by regulating energy metabolism of growth plate chondrocytes [MBD-seq]

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Bovine Hypertrophic Growth Plate Chondrocytes vs. Reserve Zone Chondrocytes

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Transcription profiling of Bos taurus hypertrophic growth plate chondrocytes vs. reserve zone chondrocytes

Project description:Growth plate chondrocytes were isolated from the distal metacarpus of young dairy cattle (all under 10 mo of age), the chondrocytes were released from the extracellular matrix by digestion with Collagenase P for 4 hours, and the various zones of the growth plate were separated by density centrifugation. The least-dense Hypertrophic Zone (HZ) cells were compared to the most-dense Reserve Zone (RZ) cells. 6 pairs of HZ vs RZ were compared by microarray. Experiment Overall Design: Growth plate chondrocytes were isolated from the distal metacarpus of young dairy cattle (all under 10 mo of age), the chondrocytes were released from the extracellular matrix by digestion with Collagenase P for 4 hours, and the various zones of the growth plate were separated by density centrifugation. The least-dense Hypertrophic Zone (HZ) cells were compared to the most-dense Reserve Zone (RZ) cells. Six independent sample pairs of HZ vs RZ were compared by microarray.

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Single cell RNAseq analysis of Col2a1+ growth plate chondrocytes

Project description:We set out to reveal single cell transcriptional profiles of neonatal growth plate chondrocytes

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Project description:We revealed a single cell transcriptional landscape of fetal growth plate chondrocytes and perichondrial cells.

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