Project description:It has been well documented that pre-eclampsia and unexplained fetal growth restriction (FGR) has a common etiological background, but little is known about the linkage al the molecular level. we have performed global gene expression profiling by oligonucleotide microarrays for placentas from pre-eclamptic, unexplained FGR and normal pregnancies to further elucidate the mechanisms underlying the development of these two disorders. The total number of samples used was 8 from pre-eclampsia, 8 from normotensive pregnancies with FGR and 8 from those without FGR.
Project description:Investigation of whole genome gene expression level changes between placentas from pregnancies with PE and placentas from normal pregnancies to search for the candiadate genes for further DNA methylation analysis The differentially expressed genes LEP and SH3PXD2A were further analyzed by DNA methylation. 5 placentas from pregnancies with PE and 7 from normal subjects were included in the gene expression microarray analysis.
Project description:Investigation of whole genome gene expression level changes between placentas from pregnancies with PE and placentas from normal pregnancies to search for the candiadate genes for further DNA methylation analysis The differentially expressed genes LEP and SH3PXD2A were further analyzed by DNA methylation.
Project description:The composition of cardiac EVs (cEVs) isolated from hypertensive mice and their capacity to reprogram cardiac fibroblasts was investigated using mass spectrometry-based proteomic profiling. Hypertensive and recovering cEVs displayed altered presence of proteins involved in peptidase activity, response to oxygen radical, and glycerolipid metabolism. Following their uptake to cardiac fibroblasts, cEV treatment from normotensive animals resulted in potent antifibrotic and prohomeostatic functions, with sex dependent differences in RNA splicing and energy metabolism proteins.Bioinformatic analysis of hypertensive-cEV remodelled fibroblasts revealed disease associated profibrotic signalling, and sex specific influences on components involved in antioxidant activity, RNA metabolism, fatty acid metabolism, and glycosylation processing. Moreover, investigation of temporal cEV signalling across hypertension and recovery revealed dynamic sex and disease dependent reprogramming of proteins related to RNA binding, interferon signalling, protein glycosylation, ECM regulation, signal transduction, and protein and energy metabolisms. We highlight the dynamic composition and signalling of cEVs in regulating fibroblast biology, reporting sex-dependent differences in cardiac physiology and hypertension.
Project description:This SuperSeries is composed of the following subset Series: GSE28283: Renal cortex microRNA expression differences between hypertensive and normotensive patients GSE28344: Renal medulla microRNA expression differences between hypertensive and normotensive patients GSE28345: Renal cortex expression differences between hypertensive and normotensive patients GSE28360: Renal medulla expression differences between hypertensive and normotensive patients Refer to individual Series
Project description:Black women have highest risk of spontaneous preterm birth (SPTB) than other race, and placental insufficiency is implicated in SPTB. We performed RNA-seq study in male and female placentas from White and Black women (self-identified) with early SPTB (< 32 weeks gestation) compared to normal pregnancies (≥ 38 weeks gestation) to assess the alterations in gene expression profiles as well as ancestry and fetal sex differences. The mid-trimester placentas (19-23 weeks gestation) were used as gestational controls.
Project description:It has been well documented that pre-eclampsia and unexplained fetal growth restriction (FGR) has a common etiological background, but little is known about the linkage al the molecular level. we have performed global gene expression profiling by oligonucleotide microarrays for placentas from pre-eclamptic, unexplained FGR and normal pregnancies to further elucidate the mechanisms underlying the development of these two disorders.