Project description:Dried polyvinyl alcohol (PVA) sponges were used for subcutaneous implantation in the mice dorsum, and were harvested on days 3, 5, 10, and 16 post-implantation.
Project description:By screening the secretomes of polymer induced Pseudomonas pseudoalcaligenes we identify a new enzyme PpEst that can degrade the co-aliphatic-aromatic polyester poly(1,4-butylene adipate-co-terephthalate) (PBAT). The discovered enzyme has predicted arylesterase activity and is induced by PBAT added to the growth medium
Project description:Although the biodegradation of biodegradable plastics in soil and compost is well-studied, there is little knowledge on the metabolic mechanisms of synthetic polymers degradation by marine microorganisms. Here, we present a multiomics study to elucidate the biodegradation mechanism of a commercial aromatic-aliphatic copolyester film by a marine microbial enrichment culture. The plastic film and each monomer can be used as sole carbon source. Our analysis showed that the consortium synergistically degrades the polymer, different degradation steps being performed by different members of the community. Analysis of gene expression and translation profiles revealed that the relevant degradation processes in the marine consortium are closely related to poly(ethylene terephthalate) biodegradation from terrestrial microbes. Although there are multiple genes and organisms with the potential to perform a degradation step, only a few of these are active during biodegradation. Our results elucidate the potential of marine microorganisms to mineralize biodegradable plastic polymers and describe the mechanisms of labor division within the community to get maximum energetic yield from a complex synthetic substrate.
Project description:We previously reported a polyvinyl alcohol-based mouse hematopoietic stem cell (HSC) culture protocol that efficiently expanded transplantable HSCs for at least a month ex vivo (Wilkinson et al., Nature 2019). Here, we investigated the molecular consequences of oxygen concentration on 28-day ex vivo HSC cultures using bulk RNA-seq
Project description:We previously reported a polyvinyl alcohol-based mouse hematopoietic stem cell (HSC) culture protocol that efficiently expanded transplantable HSCs for at least a month ex vivo (Wilkinson et al., Nature 2019). Here, we investigated the molecular consequences of oxygen concentration on 28-day ex vivo HSC cultures using single cell RNA-seq
Project description:Exoproteomes from the yeast Vishniacozyma dimennae blapla were analyzed in order to identify putative polyesterases responsible for its ability to hydrolyze various biodegradable plastics. To this end, the yeast was grown in a mineral medium and in the presence of different polyesters, like poly(caprolactone), poly(lactic acid), poly(ethylene succinate), poly(butylene succinate) and poly(butylene adipate-co-terephthalate), as well as with pyruvate and glucose as controls. The ultimate goal was to overexpress putative detected polyesterases to find the one responsible for the polyester-hydrolyzing phenotype.
Project description:This study is a prospective, multicenter, randomized controlled clinical trial to evaluate the safety and efficacy of polyvinyl alcohol sodium acrylate embolization microspheres and HepaSphere Microspheres loaded with irinotecan for the treatment of colorectal cancer with hepatic metastases through arterial chemoembolization.
