Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Precision targeting of beta-catenin induces tumor reprogramming and immunity in hepatocellular cancers [Spatial Transcriptomics by 10X Visium]

ABSTRACT: Precision targeting of beta-catenin induces tumor reprogramming and immunity in hepatocellular cancers [Spatial Transcriptomics by 10X Visium]

PROVIDER: PRJNA1129143 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR29632727_1.fastq.gz	Fastqsanger.gz
	SRR29632727_2.fastq.gz	Fastqsanger.gz
	SRR29632728_1.fastq.gz	Fastqsanger.gz
	SRR29632728_2.fastq.gz	Fastqsanger.gz
	SRR29632729_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 16

Similar Datasets

Spatial transcriptomics of gastric cancers (Visium 10X platform)

Project description:A total of 9 primary gastric cancers (1 pair of primary-metastasis, GC6) analyzed using Visium 10X platform-based spatial transcriptomics.

2023-12-26 | GSE251950 | GEO

Precision targeting of beta-catenin induces tumor reprogramming and immunity in hepatocellular cancers [Spatial Transcriptomics by 10X Visium CTNNB1/NFE2L2]

Project description:Precision targeting of beta-catenin induces tumor reprogramming and immunity in hepatocellular cancers [Spatial Transcriptomics by 10X Visium CTNNB1/NFE2L2]

| PRJNA1228034 | ENA

Spatial transcriptomics of gastric cancers (Visium 10X platform)

Project description:Spatial transcriptomics of gastric cancers (Visium 10X platform)

| PRJNA1055976 | ENA

Precision targeting of beta-catenin induces tumor reprogramming and immunity in hepatocellular cancers [Spatial Transcriptomics by 10X Visium]

Project description:First-line immune checkpoint inhibitor (ICI) combinations have shown responses in subsets of patients with advanced hepatocellular carcinoma (HCC). Nearly half of patients are Wnt active with mutations in CTNNB1 (encoding for beta-catenin), AXIN1/2, or APC, and demonstrate limited benefit to ICI due to an immunosuppressive tumor microenvironment. We investigated a novel siRNA encapsulated in lipid nanoparticle targeting CTNNB1 (LNP-CTNNB1) in multiple beta-catenin-mutated immunocompetent HCC mouse models, observing complete response. Integrated single-cell and spatial transcriptomics revealed complete cellular and zonal reprogramming of CTNNB1-mutated tumors, and a spatiotemporal shift in innate immune surveillance with intra-tumoral pro-inflammatory myeloid infiltration following LNP-CTNNB1 intervention. beta-catenin knockdown initiated tumor cell-intrinsic type I/II interferon signaling accompanied by IRF2 upregulation, which was suppressed in CTNNB1-mutated HCCs. IRF2 re-expression in beta-catenin-mutated HCC restored adaptive tumor immunity with increased CD4+ T cells and impaired recruitment of resident T regulatory cells. Finally, LNP-CTNNB1 synergized with ICI in advanced-stage disease through increasing intra-tumoral recruitment of cytotoxic T cells. In summary, RNAi-mediated inhibition of CTNNB1 is efficacious as monotherapy and in combination with ICI in CTNNB1-mutated HCCs through remodeling both tumor cell intrinsic signaling and global immune surveillance, thus providing rationale for clinical investigations.

2025-05-02 | GSE270975 | GEO

Spatial transcriptomics (10x Visium) of healthy and trisomy 21 (Down's syndrome) foetal liver

Project description:We performed 10X Visium on two consecutive 10 µm sections from 11 Ts21 foetal livers and five healthy foetal livers.

2024-07-17 | E-MTAB-13062 | biostudies-arrayexpress

Spatial transcriptomics (10x Visium) of human NSCLC lesions and non-involved tissue

Project description:We performed 10X Visium on consecutive 10 µm sections from 8 human NSCLC tumours (total 20 sections), 8 adjacent non-involved lung tissues (total 16 sections) and two healthy donors (total 4 sections)

2024-04-29 | E-MTAB-13530 | biostudies-arrayexpress

Precision targeting of beta-catenin induces tumor reprogramming and immunity in hepatocellular cancers [Spatial Transcriptomics by 10X Visium CTNNB1/NFE2L2]

2025-05-02 | GSE290445 | GEO

Comparing 10x Visium spatial transcriptomic technologies

Project description:Comparing 10x Visium spatial transcriptomic technologies

| PRJNA1071205 | ENA

Comparing 10x Visium spatial transcriptomic technologies

Project description:Spatial transcriptomics facilitates the understanding of gene expression within complex tissue contexts. Among the array of spatial capture technologies available is 10x Genomics’ Visium which provides whole tissue section profiling, enabling whole transcriptome spatial analysis. Our dataset comprises spleen tissue from mice infected with malaria, spanning multiple experiments and sample preparation protocols for tissue preservation, either as fresh frozen at optimal cutting temperature (OCT) or formalin-fixed paraffin-embedded (FFPE). Tissue placement was also considered, comparing direct tissue placement on the slide with the use of CytAssist (CA), which expands the Visium platform’s capabilities by allowing for the pre-selection of tissue sections and genes through a set of probes. We also include a matching scRNA-seq dataset that can be integrated with the spatial data.

2024-02-26 | GSE254652 | GEO

Precision targeting of beta-catenin induces tumor reprogramming and immunity in hepatocellular cancers

Project description:Precision targeting of beta-catenin induces tumor reprogramming and immunity in hepatocellular cancers

| PRJNA1129135 | ENA