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Mex3a-dependent post-transcriptional silencing assures olfactory receptor diversity and axon guidance specificity [scRNA-Seq]

ABSTRACT: Mex3a-dependent post-transcriptional silencing assures olfactory receptor diversity and axon guidance specificity [scRNA-Seq]

PROVIDER: PRJNA1129446 | ENA |

REPOSITORIES: ENA

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Mex3a-dependent post-transcriptional silencing assures olfactory receptor diversity and axon guidance specificity [scRNA-Seq]

Project description:Singular olfactory receptor (OR) expression is critical for vertebrate olfaction because the sequence of the chosen OR protein determines both the receptive field and axon guidance properties of each olfactory sensory neuron (OSN). However, numerous single cell RNA-seq studies reveal that OSN progenitors co-transcribe seemingly random combinations of OR genes before a gradual transition to transcriptional singularity. Here, we show that MEX3A, a RING and KH domain protein expressed during polygenic OR transcription, prevents efficient OR protein expression before the emergence of a prevalent, transcriptionally dominant OR allele. Consequently, MEX3A deletion enhances OR mRNA and OR protein abundance in OSN progenitors, resulting in premature induction of Perk signaling, biased OR gene choice, and OSN axon mistargeting. Our experiments uncover a novel, post-transcriptional mechanism of OR gene regulation that assures that the identity of the expressed OR can influence the identity of the OSN only at the onset of singular OR choice.

2025-05-09 | GSE271031 | GEO

Mex3a-dependent post-transcriptional silencing assures olfactory receptor diversity and axon guidance specificity [RNA-Seq]

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Mex3a-dependent post-transcriptional silencing assures olfactory receptor diversity and axon guidance specificity [Ribo-Seq]

2025-05-09 | GSE271027 | GEO

Mex3a-dependent post-transcriptional silencing assures olfactory receptor diversity and axon guidance specificity [RNA-Seq]

Project description:Mex3a-dependent post-transcriptional silencing assures olfactory receptor diversity and axon guidance specificity [RNA-Seq]

| PRJNA1129448 | ENA

Mex3a-dependent post-transcriptional silencing assures olfactory receptor diversity and axon guidance specificity [Ribo-Seq]

Project description:Mex3a-dependent post-transcriptional silencing assures olfactory receptor diversity and axon guidance specificity [Ribo-Seq]

| PRJNA1129445 | ENA

Distinct, OR-specific subsets of axon guidance molecules govern early olfactory map formation

Project description:We have discovered subsets of axon guidance molecules and transcription factors that are enriched in specific subsets of olfactory sensory neurons. We have demonstrated guidance activity for three of the candidate axon guidance genes we identified, suggesting that this approach is an efficient method for characterizing guidance systems relevant to olfactory axon targeting.

2018-02-19 | GSE103692 | GEO

ER stress transforms stochastic olfactory receptor gene choice into stereotypic axon guidance programs

Project description:Olfactory sensory neurons (OSNs) transform the stochastic choice of one out of >1000 olfactory receptor (OR) genes into precise and stereotyped axon targeting of OR-specific glomeruli in the olfactory bulb. Here, we show that the PERK arm of the unfolded protein response (UPR) regulates both the glomerular coalescence of like axons, and the specificity of their projections. Subtle differences in OR protein sequences lead to distinct patterns of endoplasmic reticulum (ER) stress during OSN development, converting OR identity into distinct gene expression signatures. We identify the transcription factor Ddit3 as a key effector of PERK signaling that maps OR-dependent ER signaling patterns to the transcriptional regulation of axon guidance and cell adhesion genes, instructing targeting precision. Our results extend the known functions of the UPR from a quality control pathway that protects cells from misfolded proteins, to a sensor of cellular identity that interprets physiological states to direct axon wiring.

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Identification of a microRNA regulator for axon guidance in the olfactory bulb of adult mice

Project description:Using the highly sensitive miRNA array, we screened 40 microRNAs abundant in the olfactory bulb and we explored the functions of these miRNAs in the olfactory bulb by Gene Ontology and Kyoto Encyclopedia of Genes annotation. The enrichment results indicated that these miRNAs mainly participated in the axon guidance process. Furthermore, the quantitative real-time polymerase chain reaction, immunohistochemistry, and dual luciferase reporter assay results showed that miR-30c is a specific regulator of semaphorin-3A, which will give new insights in disclosing the mechanism of functional maintenance and sexual-specific differentiation of the olfactory bulb. In this study, three samples from steady-state mice were used to acquire the miRNA expression profiling and the function of the abudant miRNAs in the olfactory bulb were analyzed by bioinformatic methods.Finally, miR-30c was experimentally validated to be a regulator of semaphorin-3A, an important axon guidance cue in the nervous system.

2014-08-27 | E-GEOD-55463 | biostudies-arrayexpress