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Effect of Matrigel, Laminin and Poly-L-Ornithine coating substrates on primary enteric glial cells and comparison to in vivo enteric glia

ABSTRACT: Effect of Matrigel, Laminin and Poly-L-Ornithine coating substrates on primary enteric glial cells and comparison to in vivo enteric glia

PROVIDER: PRJNA1129996 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR29655205.fastq.gz	Fastqsanger.gz
	SRR29655206.fastq.gz	Fastqsanger.gz
	SRR29655207.fastq.gz	Fastqsanger.gz
	SRR29655208.fastq.gz	Fastqsanger.gz
	SRR29655209.fastq.gz	Fastqsanger.gz

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Project description:Enteric glial cells are important players in the control of motility, intestinal barrier integrity and inflammation. During inflammation, they switch into a reactive phenotype enabling them to release inflammatory mediators, thereby shaping the inflammatory environment. While a plethora of well-established in vivo models exist, cell culture models necessary to decipher the mechanistic pathways of enteric glial reactivity are less well standardized. In particular, the composition of extracellular matrices (ECM) can massively affect the experimental outcome. Considering the growing number of studies involving primary enteric glial cells, a better understanding of their homeostatic and inflammatory in vitro culture conditions is needed. We examined the impact of different ECMs on enteric glial culture homeostasis and immune responsiveness. We used 3’ bulk mRNA sequencing to compare cultured cells with in vivo enteric glial transcriptomes isolated from Sox10iCreERT2Rpl22HA/+ mice. Transcriptional analysis revealed strong similarities between enteric glia on Matrigel and laminin with enrichment of gene sets supporting neuronal differentiation, while cells on poly-L-ornithine showed enrichment related to cell proliferation. Comparing cultured and in vivo enteric glial transcriptomes revealed a 50% overlap. IL-1β treatment showed distinct coating-dependent gene expression signatures, with an enrichment of genes related to myeloid and epithelial cell differentiation on Matrigel and laminin coatings, while poly-L-ornithine induced more gene sets related to lymphocyte differentiation. Together, changes in differentiation and immune activation of primary enteric glial cells proved a strong effect of the ECM. We identified Matrigel and laminin as pre-eminent substrates for murine enteric glial cultures. These new insights will help to standardize and improve enteric glial culture quality and reproducibility between studies in the future, allowing a better comparison of their functional role in enteric neuroinflammation.

Dataset Information

Effect of Matrigel, Laminin and Poly-L-Ornithine coating substrates on primary enteric glial cells and comparison to in vivo enteric glia

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