Project description:The specificity of the RNA-CASing process was analysed by Next-Generation Sequencing. Therfor small RNAs were isolated from purified proteins of Escherichia coli and subjected to Illumina sequencing or nanopore sequencing.
Project description:Metagenomics analysis reveals co-infection of fungi and bacteria isolated from different regions of brain tissue from elderly persons and patients with Alzheimer's disease.
Project description:This study looks at the gene expression of two chain-elongating bacteria, Pseudoramibacter alactolyticus and Megasphaera elsdenii, in monoculture and in coculture with anaerobic gut fungi (Neocallimastix lanati). P. alactolyticus in particular has appeared stable in synthetic cocultures with anaerobic fungi based on metabolic output, and this RNA-seq study was designed to further assess that stability. Additionally, chain-elongating bacteria can use fungal fermentation products to generate short- and medium-chain fatty acids, and this study was employed to see how genes involved in this process (reverse beta oxidation) were regulated in coculture compared to monoculture.